Mesenchymal Stem Cells

Methods and Protocols

  • Massimiliano Gnecchi

Part of the Methods in Molecular Biology book series (MIMB, volume 1416)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Overview of Mesenchymal Stem Cells For Cell Therapy

    1. Front Matter
      Pages 1-1
    2. Maria Ester Bernardo, Franco Locatelli
      Pages 3-20
    3. Rodolfo Quarto, Paolo Giannoni
      Pages 21-33
    4. Johnathan Ng, Jonathan Bernhard, Gordana Vunjak-Novakovic
      Pages 35-54
    5. Ian A. White, Cristina Sanina, Wayne Balkan, Joshua M. Hare
      Pages 55-87
    6. Marina Morigi, Cinzia Rota, Giuseppe Remuzzi
      Pages 89-107
    7. Carlo Tremolada, Camillo Ricordi, Arnold I. Caplan, Carlo Ventura
      Pages 109-122
    8. Massimiliano Gnecchi, Patrizia Danieli, Giuseppe Malpasso, Maria Chiara Ciuffreda
      Pages 123-146
  3. Isolation and Characterization of Mesenchymal Stem Cells

    1. Front Matter
      Pages 147-147
    2. Maria Chiara Ciuffreda, Giuseppe Malpasso, Paola Musarò, Valentina Turco, Massimiliano Gnecchi
      Pages 149-158
    3. Patrice Penfornis, Radhika Pochampally
      Pages 159-169
    4. R. Wilder Scott, T. Michael Underhill
      Pages 171-203
    5. Siddaraju V. Boregowda, Veena Krishnappa, Donald G. Phinney
      Pages 205-223
    6. Dries A. M. Feyen, Frederieke van den Akker, Willy Noort, Steven A. J. Chamuleau, Pieter A. Doevendans, Joost P. G. Sluijter
      Pages 225-232
    7. Minh Quan Vu, Shant Der Sarkissian, Melanie Borie, Pierre-Olivier Bessette, Nicolas Noiseux
      Pages 275-288
    8. Qizhou Lian, Yuelin Zhang, Xiaoting Liang, Fei Gao, Hung-Fat Tse
      Pages 289-298
  4. Mesenchymal Stem Cells for Clinical Use

    1. Front Matter
      Pages 299-299
    2. Marina Radrizzani, Sabrina Soncin, Viviana Lo Cicero, Gabriella Andriolo, Sara Bolis, Lucia Turchetto
      Pages 313-337
    3. Marina Radrizzani, Sabrina Soncin, Sara Bolis, Viviana Lo Cicero, Gabriella Andriolo, Lucia Turchetto
      Pages 339-356
    4. Mandana Haack-Sørensen, Annette Ekblond, Jens Kastrup
      Pages 357-374
    5. Ana Fernandes-Platzgummer, Joana G. Carmelo, Cláudia Lobato da Silva, Joaquim M. S. Cabral
      Pages 375-388
    6. Christina Barckhausen, Brent Rice, Stefano Baila, Luc Sensebé, Hubert Schrezenmeier, Philipp Nold et al.
      Pages 389-412
    7. Celeste Scotti, Beatrice Tonnarelli, Adam Papadimitropoulos, Elia Piccinini, Atanas Todorov, Matteo Centola et al.
      Pages 413-424
  5. Mesenchymal Stem Cell Secretome

    1. Front Matter
      Pages 443-443
    2. Patrizia Danieli, Giuseppe Malpasso, Maria Chiara Ciuffreda, Massimiliano Gnecchi
      Pages 445-456
    3. Ruenn Chai Lai, Ronne Wee Yeh Yeo, Jayanthi Padmanabhan, Andre Choo, Dominique P. V. de Kleijn, Sai Kiang Lim
      Pages 477-494
    4. Jose Gomez, Jeffrey Schmeckpeper, Maria Mirotsou
      Pages 495-507
    5. Vitor Marcel Faça, Maristela Delgado Orellana, Lewis Joel Greene, Dimas Tadeu Covas
      Pages 509-519
    6. Sandra I. Anjo, Ana S. Lourenço, Matilde N. Melo, Cátia Santa, Bruno Manadas
      Pages 521-549
    7. Beatriz Rocha, Valentina Calamia, Francisco J. Blanco, Cristina Ruiz-Romero
      Pages 551-565
  6. Back Matter
    Pages 567-570

About this book


This volume aims to outline the current status of the Mesenchymal Stem Cells(MSC) field in regenerative medicine and to propose clear and reproducible protocols to better define the identity, function and use of these cells that are today, more than ever, “under the spotlight”. Mesenchymal Stem Cells: Methods and Protocols, Second Edition is organized into four sections. The first guides the reader through a series of state-of-the-art reviews summarizing the use of MSC for the treatment of various diseases. The other three sections are a collection of methodological chapters covering several aspects: isolation and characterization of MSC; expansion of MSC for clinical use; production and characterization of the MSC secretome. Written in the highly successful Methods in Molecular Biology series format, the method chapters include introductions to their respective topics, complete lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting which will help the researcher to avoid known pitfalls.

Authoritative and cutting-edge, Mesenchymal Stem Cells: Methods and Protocols, Second Edition, aims to ensure successful results in the further study of this vital field.


Mesenchymal stem cells (MSC) cell therapy isolation characterization MSC secretome clinical use

Editors and affiliations

  • Massimiliano Gnecchi
    • 1
  1. 1.Dept of Molecular Med,Unit of CardiologyUniversity of PaviaPAVIAItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3582-6
  • Online ISBN 978-1-4939-3584-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site