Abstract
Protein-protein interactions play central roles in intercellular and intracellular signal transduction. Impairment of protein-protein interactions causes many diseases such as cancer, cardiomyopathies, diabetes, microbial infections, and genetic and neurodegenerative disorders. Immunoprecipitation is a technique in which a target protein of interest bound by an antibody is used to pull down the protein complex out of cell lysates, which can be identified by mass spectrometry. Here, we describe the protocol to immunoprecipitate and identify the components of the protein complexes of ElmoE in Dictyostelium discoideum cells.
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Acknowledgement
This work was supported by grants of National Basic Research Program of China (2014CB541804), Shanghai Pujiang Program (14PJ1407700), and Health and Family Planning Commission Foundation of Shanghai (201440300) to J.Y. and by National Nature Science Foundation of China (81201977) to X.C.
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Cao, X., Yan, J. (2016). Identification of Associated Proteins by Immunoprecipitation and Mass Spectrometry Analysis. In: Jin, T., Hereld, D. (eds) Chemotaxis. Methods in Molecular Biology, vol 1407. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3480-5_10
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DOI: https://doi.org/10.1007/978-1-4939-3480-5_10
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3480-5
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