Methods and Protocols

  • Tian Jin
  • Dale Hereld

Part of the Methods in Molecular Biology book series (MIMB, volume 1407)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. David E. Stone, Robert A. Arkowitz
    Pages 1-12
  3. Allison W. McClure, Chi-Fang Wu, Sam A. Johnson, Daniel J. Lew
    Pages 13-23
  4. Karl J. Aufderheide, Chris Janetopoulos
    Pages 25-39
  5. Sui Lay, Oana Sanislav, Sarah J. Annesley, Paul R. Fisher
    Pages 41-61
  6. A. F. M. Tariqul Islam, Branden M. Stepanski, Pascale G. Charest
    Pages 63-77
  7. Evgeny Zatulovskiy, Robert R. Kay
    Pages 97-105
  8. Grant P. Otto, Marco Cocorocchio, Laura Munoz, Richard A. Tyson, Till Bretschneider, Robin S. B. Williams
    Pages 123-130
  9. Wei Dai, Denise J. Montell
    Pages 153-168
  10. Abdul Basit, Wenwen Tang, Dianqing Wu
    Pages 169-177
  11. Yoshishige Miyabe, Nancy D. Kim, Chie Miyabe, Andrew D. Luster
    Pages 179-194
  12. Olivia Susanto, Andrew J. Muinonen-Martin, Max Nobis, Robert H. Insall
    Pages 217-228
  13. Spencer Kuhl, Edward Voss, Amanda Scherer, Daniel F. Lusche, Deborah Wessels, David R. Soll
    Pages 229-250
  14. Yao-Hui Sun, Yuxin Sun, Kan Zhu, Bruce W. Draper, Qunli Zeng, Alex Mogilner et al.
    Pages 251-272

About this book


This second edition expands on the first edition with new chapters describing methods for studying cell movement, molecular components involved in chemotaxis, spatiotemporal dynamics of signaling components, and quantitative modeling, as well as several updated chapters from the first edition. Various methods to investigate directional cell growth and movements are presented in Chapters 1-20. These chapters contains experimental procedures to visualize and measure migration behaviors of different kinds of organisms, including chemotropism in the budding yeast; cell growth and migration of D. discoideum; border cell migration in Drosophila; chemotaxis of mouse and human neutrophils; and HIV-induced T cell chemotactic response.Chemotaxis: Methods and Protocols, Second Edition also contains microscopy procedures for studying breast cancer cell migration, tumor cell invasion in vivo, and axon guidance. The book concludes with Chapters 21-27 describing methods that measure spatiotemporal dynamics of signaling components involved in chemotaxis; introduce imaging techniques, such as TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models of experimentally generated chemoattractant gradients. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Chemotaxis: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the diverse methodologies that are propelling chemotaxis research forward. 


cell movement spatiotemporal dynamics of signaling components quantitative modeling directional cell growth breast cancer cell migration tumor call invasion in vivo axon guidance single-molecule microscopy

Editors and affiliations

  • Tian Jin
    • 1
  • Dale Hereld
    • 2
  1. 1.Nat Inst of Allergy& Infectious Dis, NIHLaboratory of ImmunogeneticsRockvilleUSA
  2. 2.National Institutes of HealthNat Inst Alcohol Abuse & AlcoholismRockvilleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3478-2
  • Online ISBN 978-1-4939-3480-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book