Posttranscriptional control makes an important contribution to circadian regulation of gene expression. In higher plants, alternative splicing is particularly prevalent upon abiotic and biotic stress and in the circadian system. Here we describe in detail a high-resolution reverse transcription-PCR based panel (HR RT-PCR) to monitor alternative splicing events. The use of the panel allows the quantification of changes in the proportion of splice isoforms between different samples, e.g., different time points, different tissues, genotypes, ecotypes, or treatments.
Alternative splicing Circadian rhythm RNA-binding proteins Splicing factors
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Work in our laboratories is supported by grants from the Biotechnology and Biological Sciences Research Council (BB/G024979/1, European Research Area network Plant Genomics [Plant Alternative Splicing and Abiotic Stress]) and the Scottish Government Rural and Environment Science and Analytical Services division (to J.W.S.B. and C.S.) and the DFG (STA 653 and SPP1530) (to D.S.).
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