Book Volume 1398 2016

Environmental Responses in Plants

Methods and Protocols

Editors:

ISBN: 978-1-4939-3354-9 (Print) 978-1-4939-3356-3 (Online)

Table of contents (30 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Tropisms

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-9

      Hydrotropism: Analysis of the Root Response to a Moisture Gradient

    3. No Access

      Protocol

      Pages 11-20

      Assessing Gravitropic Responses in Arabidopsis

    4. No Access

      Protocol

      Pages 21-28

      Physiological Analysis of Phototropic Responses in Arabidopsis

    5. No Access

      Protocol

      Pages 29-35

      Automatic Chloroplast Movement Analysis

    6. No Access

      Protocol

      Pages 37-53

      Microscopic and Biochemical Visualization of Auxins in Plant Tissues

    7. No Access

      Protocol

      Pages 55-67

      Immunolocalization of PIN and ABCB Transporters in Plants

  3. Photoperiodism and Circadian Rhythms

    1. Front Matter

      Pages 69-69

    2. No Access

      Protocol

      Pages 71-79

      Analysis of Circadian Leaf Movements

    3. No Access

      Protocol

      Pages 81-91

      Sample Preparation of Arabidopsis thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes

    4. No Access

      Protocol

      Pages 93-106

      A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs Are Temporally Restricted by the Circadian Clock

    5. No Access

      Protocol

      Pages 107-118

      Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hybrid Screens Using a Transcription Factor ORFeome

    6. No Access

      Protocol

      Pages 119-132

      Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes

    7. No Access

      Protocol

      Pages 133-140

      Assessing the Impact of Photosynthetic Sugars on the Arabidopsis Circadian Clock

    8. No Access

      Protocol

      Pages 141-152

      Assessing Protein Stability Under Different Light and Circadian Conditions

  4. Abiotic Stress Responses

    1. Front Matter

      Pages 153-153

    2. No Access

      Protocol

      Pages 155-182

      Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought

    3. No Access

      Protocol

      Pages 183-196

      Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis thaliana

    4. No Access

      Protocol

      Pages 197-208

      Assessing Tolerance to Heavy-Metal Stress in Arabidopsis thaliana Seedlings

    5. No Access

      Protocol

      Pages 209-219

      Assessing Drought Responses Using Thermal Infrared Imaging

    6. No Access

      Protocol

      Pages 221-234

      Generating Targeted Gene Knockout Lines in Physcomitrella patens to Study Evolution of Stress-Responsive Mechanisms

    7. No Access

      Protocol

      Pages 235-246

      Screening Stress Tolerance Traits in Arabidopsis Cell Cultures

    8. No Access

      Protocol

      Pages 247-269

      Using Arabidopsis Protoplasts to Study Cellular Responses to Environmental Stress

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