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Methods to Investigate the Role of Toll-Like Receptors in Allergic Contact Dermatitis

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Toll-Like Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1390))

Abstract

Allergic contact disease is a common inflammatory skin disease resulting from hyperresponsiveness to harmless nonprotein substances such as metals, fragrances, or rubber. Recent research has highlighted a prominent role of Toll-like receptors, particularly TLR4 in contact allergen-induced innate immune activation that crucially contributes to the pathogenesis of this disease.

Here we describe several methods to investigate the role of Toll-like receptors in contact allergen-induced pro-inflammatory responses. These include expansion of disease-relevant human primary cells including endothelial cells and keratinocytes and their manipulation of TLR signaling by transfection, retroviral infection and RNA interference, basic methods to induce contact hypersensitivity in mice, and protocols for adoptive transfer of hapten-stimulated dendritic cells and T cells from TLR-deficient mice to wild-type mice and vice versa wild-type mice to TLR-deficient mice in order to explore cell-specific roles of TLRs in contact hypersensitivity responses.

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Author information

Authors and Affiliations

  1. Department of Dermatology, University of Würzburg, Josef-Schneider Str. 2, Würzburg, 97080, Germany

    Marc Schmidt & Matthias Goebeler

  2. Department of Dermatology, University Medical Center Freiburg, Hauptstraße 7, Freiburg, 79104, Germany

    Stefan F. Martin

Authors
  1. Marc Schmidt
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  2. Matthias Goebeler
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  3. Stefan F. Martin
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Corresponding author

Correspondence to Marc Schmidt .

Editor information

Editors and Affiliations

  1. Hudson Institute of Medical Research, Monash University Faculty of Medicine, Clayton, Victoria, Australia

    Claire E. McCoy

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Schmidt, M., Goebeler, M., Martin, S.F. (2016). Methods to Investigate the Role of Toll-Like Receptors in Allergic Contact Dermatitis. In: McCoy, C. (eds) Toll-Like Receptors. Methods in Molecular Biology, vol 1390. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3335-8_20

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  • DOI: https://doi.org/10.1007/978-1-4939-3335-8_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3333-4

  • Online ISBN: 978-1-4939-3335-8

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