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Induction of Contact Hypersensitivity in the Mouse Model

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Molecular Dermatology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 961))

Abstract

Contact hypersensitivity (CHS) in the mouse model is a standard method to assess delayed type hypersensitivity (DTH) responses in the skin induced by low molecular weight chemicals that in humans cause contact dermatitis. These responses are clinically important and present as eczematous skin reactions. Here, this chapter describes the standard protocol for T cell-mediated CHS and a variation thereof, which allows to address more specific questions regarding immunologic pathomechanisms.

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Abbreviations

ACD:

Allergic contact dermatitis

AOO:

Acetone/olive oil

ADBP:

Acetone/dibutyl phthalate

CHS:

Contact hypersensitivity

DC:

Dendritic cell

DNFB:

2,4-Dinitrofluorobenzene

DNBS:

2,4-Dinitrobenzene sulfonic acid

DMSO:

Dimethylsulfoxide

DMF:

Dimethylformamide

DTH:

Delayed type hypersensitivity

FITC:

Fluorescein isothiocyanate

LLNA:

Local lymph node assay

MEST:

Mouse ear swelling test

TNCB:

2,4,6-Trinitrochlorobenzene

TNBS:

2,4,6-Trinitrobenzene sulfonic acid

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Acknowledgment

The author’s work referenced here was supported in part by grants of the European Commission as part of the project “Novel Testing Strategies for In Vitro Assessment of Allergens (Sens-it-iv)” (LSHB-CT-2005-018681) (see http://www.sens-it-iv.eu), by the Deutsche Forschungsgemeinschaft (DFG) and by the Research Commission of the Medical Faculty of the University Freiburg Medical Center.

Dr. Philipp Esser is acknowledged for careful reading of the manuscript.

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Authors and Affiliations

  1. Allergy Research Group, Department of Dermatology, University Freiburg Medical Center, Freiburg, Germany

    Stefan F. Martin

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  1. Stefan F. Martin
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Correspondence to Stefan F. Martin .

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Editors and Affiliations

  1. Universitäts-Hautklinik, Department of Molecular Dermatology, Universitätsklinikum Freiburg, Hauptstr. 7, Freiburg, 79104, Germany

    Cristina Has

  2. Universitäts-Hautklinik, Department of Molecular Dermatology, Universitätklinikum Freiburg, Hauptstr. 7, Freiburg, 79104, Germany

    Cassian Sitaru

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Martin, S.F. (2013). Induction of Contact Hypersensitivity in the Mouse Model. In: Has, C., Sitaru, C. (eds) Molecular Dermatology. Methods in Molecular Biology, vol 961. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-227-8_21

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  • DOI: https://doi.org/10.1007/978-1-62703-227-8_21

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-226-1

  • Online ISBN: 978-1-62703-227-8

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