Skip to main content

Computational Classification of P-Type ATPases

  • Protocol
P-Type ATPases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1377))

  • 3270 Accesses

Abstract

Analysis of sequence data is inevitable in modern molecular biology, and important information about for example proteins can be inferred efficiently using computational methods. Here, we explain how to use the information in freely available databases together with computational methods for classification and motif detection to assess whether a protein sequence corresponds to a P-type ATPase (and if so, which subtype) or not.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. The UniProt Consortium (2008) The Universal Protein Resource (UniProt). Nucleic Acids Res 36:D190–D195

    Article  PubMed Central  Google Scholar 

  2. Sigrist CJA, Cerutti L, Hulo N, Gattiker A, Falquet L, Pagni M, Bairoch A, Bucher P (2002) PROSITE: a documented database using patterns and profiles as motif descriptors. Brief Bioinform 3(3):265–274

    Article  PubMed  CAS  Google Scholar 

  3. Saier MH Jr, Tran CV, Barabote RD (2006) TCDB: the transporter classification database for membrane transport protein analyses and information. Nucleic Acids Res 34:D181–D186

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  4. Pedersen BP, Ifrim G, Liboriussen P, Axelsen KB, Palmgren MG, Nissen P, Wiuf C, Pedersen CNS (2014) Large scale identification and categorization of protein sequences using structured logistic regression. PLoS One 9(1): e85139

    Article  PubMed  PubMed Central  Google Scholar 

  5. Axelsen KB, Palmgren MG (1998) Evolution of substrate specificities in the P-type ATPase superfamily. J Mol Evol 46:84–101

    Article  PubMed  CAS  Google Scholar 

  6. Møller AB, Asp T, Holm PB, Palmgren MG (2008) Phylogenetic analysis of P5 P-type ATPases, a eukaryotic lineage of secretory pathway pumps. Mol Phylogenet Evol 46(2):619–634

    Article  PubMed  Google Scholar 

  7. Ifrim G, Wiuf C (2011) Bounded coordinate-descent for biological sequence classification in high dimensional predictor space. Proceedings of the 17th ACM SIGKDD international conference on knowledge discovery and data mining, 708–716

    Google Scholar 

  8. Fagan MJ, Saier MH Jr (1994) P-type ATPases of eukaryotes and bacteria: sequence analyses and construction of phylogenetic trees. J Mol Evol 35:57–99

    Google Scholar 

  9. Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K, Madden TL (2009) BLAST+: architecture and applications. Bioinformatics 10:421

    PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

We wish to thank Marco Palos Franco, Bioinformatics Research Centre (BiRC), Aarhus University, for collecting and curating the P-type ATPase dataset.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Christian Nørgaard Storm Pedersen .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media New York

About this protocol

Cite this protocol

Søndergaard, D., Knudsen, M., Pedersen, C.N.S. (2016). Computational Classification of P-Type ATPases. In: Bublitz, M. (eds) P-Type ATPases. Methods in Molecular Biology, vol 1377. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3179-8_41

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-3179-8_41

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3178-1

  • Online ISBN: 978-1-4939-3179-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics