P-Type ATPases

Methods and Protocols

  • Maike Bublitz

Part of the Methods in Molecular Biology book series (MIMB, volume 1377)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Protein Production, Isolation, Purification, and Stabilization

    1. Front Matter
      Pages 3-3
    2. Natalya U. Fedosova
      Pages 5-10
    3. Xiangyu Liu, Oleg Sitsel, Kaituo Wang, Pontus Gourdon
      Pages 29-36
    4. Hassina Azouaoui, Cédric Montigny, Aurore Jacquot, Raphaëlle Barry, Philippe Champeil, Guillaume Lenoir
      Pages 37-55
    5. Ilse Vandecaetsbeek, Peter Vangheluwe
      Pages 79-85
  3. Activity Assays

    1. Front Matter
      Pages 87-87
    2. Kathleen J. Sweadner
      Pages 89-104
    3. Pankaj Sehgal, Claus Olesen, Jesper V. Møller
      Pages 105-109
    4. Gianluca Bartolommei, Francesco Tadini-Buoninsegni
      Pages 111-120
    5. Herman G. P. Swarts, Jan B. Koenderink
      Pages 121-126
    6. Hans-Jürgen Apell, Bojana Damnjanovic
      Pages 127-156
    7. Pankaj Sehgal, Claus Olesen, Jesper V. Møller
      Pages 157-160
    8. Philip A. Bidwell, Evangelia G. Kranias
      Pages 161-170
    9. Alex Green Wielandt, Michael G. Palmgren, Anja Thoe Fuglsang, Thomas Günther-Pomorski, Bo Højen Justesen
      Pages 171-180

About this book

Introduction

This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases.  P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all researchers in the P-type ATPase field, from the protein biochemist to the mouse geneticist, covering the identification and classification, isolation, purification, in vitro characterization, knock-out studies, as well as crystallization and structural analysis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, P-Type ATPases: Methods and Protocols aims to ensure successful results in the further study of this vital field of research.

Keywords

P-type ATPases crystallization electrophysiology enzyme kinetics enzymes in vitro characterization lipid membrane membrane proteins

Editors and affiliations

  • Maike Bublitz
    • 1
  1. 1.Department of Biochemistry, BiocenterUniversity of OxfordOxfordUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3179-8
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3178-1
  • Online ISBN 978-1-4939-3179-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book