Abstract
The Gli transcription factors (Gli1, Gli2, and Gli3) are the final effectors of the Hedgehog (Hh) signaling and play a key role in development and cancer. The activity of the Gli proteins is finely regulated by covalent modifications, such as phosphorylation, ubiquitination, and acetylation. Both Gli1 and Gli2 are acetylated at a conserved lysine, and this modification causes the inhibition of their transcriptional activity. Thus, the acetylation status of these proteins represents a useful marker to monitor Hh activation in pathophysiological conditions. Herein we describe the techniques utilized to detect in vitro and intracellular acetylation of the Gli transcription factors.
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Acknowledgements
This work was supported by AIRC (Associazione Italiana Ricerca Cancro) grant # IG10610, MIUR FIRB and PRIN projects, Ministry of Health, EU HEALING grant # 238186, Pasteur Institute-Cenci Bolognetti Foundation, and Italian Institute of Technology (IIT), NIH P41 RR011823. Laura Di Magno (LDM) and Sonia Coni (SC) were supported by fellowships from the Pasteur Institute-Cenci Bolognetti Foundation.
All authors disclose no financial conflict of interest that might be construed to influence the results or interpretation of their manuscript.
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Coni, S., Di Magno, L., Canettieri, G. (2015). Determination of Acetylation of the Gli Transcription Factors. In: Riobo, N. (eds) Hedgehog Signaling Protocols. Methods in Molecular Biology, vol 1322. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2772-2_13
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DOI: https://doi.org/10.1007/978-1-4939-2772-2_13
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2771-5
Online ISBN: 978-1-4939-2772-2
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