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Other Notable Methods of Membrane Protein Detection: A Brief Review

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Detection of Blotted Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1314))

Abstract

Several techniques have been employed to detect proteins on membranes. These include the use of quantum dot luminescent labels, oxyblot immunochemical detection, polymer immunocomplexes, “coupled” probing approach, in situ renaturation of proteins for detecting enzyme activities in crude or purified preparations, immunochromatographic assay, western-phosphatase assay, and use of Congo red dye (a cosmetic color named Alta), Pro-Q Emerald 488 dye, or amine-reactive dye in combination with alkaline phosphatase- and horseradish peroxidase-antibody conjugates for the simultaneous trichromatic fluorescence detection of proteins. Several methods have been used to improve the detection of proteins on membranes, including glutaraldehyde treatment of nitrocellulose blots, elimination of keratin artifacts in immunoblots probed with polyclonal antibodies, and washing of immunoblots with excessive water and manipulation of Tween-20 in wash buffer. These methods are briefly reviewed in this chapter.

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Authors and Affiliations

  1. Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    Biji T. Kurien Ph.D. & R. Hal Scofield M.D.

  2. Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA

    Biji T. Kurien Ph.D. & R. Hal Scofield M.D.

  3. Department of Veterans Affairs Medical Center, Oklahoma City, OK, USA

    Biji T. Kurien Ph.D. & R. Hal Scofield M.D.

Authors
  1. Biji T. Kurien Ph.D.
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  2. R. Hal Scofield M.D.
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Corresponding author

Correspondence to Biji T. Kurien Ph.D. .

Editor information

Editors and Affiliations

  1. Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA

    Biji T. Kurien

  2. Department of Medicine, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA

    R. Hal Scofield

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Kurien, B.T., Scofield, R.H. (2015). Other Notable Methods of Membrane Protein Detection: A Brief Review. In: Kurien, B., Scofield, R. (eds) Detection of Blotted Proteins. Methods in Molecular Biology, vol 1314. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2718-0_36

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  • DOI: https://doi.org/10.1007/978-1-4939-2718-0_36

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2717-3

  • Online ISBN: 978-1-4939-2718-0

  • eBook Packages: Springer Protocols

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