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A Brief Review of Other Notable Protein Detection Methods on Blots

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Protein Blotting and Detection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 536))

Summary

Several methods have been used for detecting proteins on membranes. These include the use of quantum dot luminescent labels, oxyblot immunochemical detection, polymer immunocomplexes, “coupled” probing approach, in situ renaturation of proteins for detecting enzyme activities in crude or purified preparations, immunochromatographic assay, western-phosphatase assay, and the use of Congo red dye, a cosmetic color named Alta, Pro-Q Emerald 488 dye, or amine-reactive dye in combination with alkaline phosphatase and horseradish peroxidase–antibody conjugates for the simultaneous trichromatic fluourescence detection of proteins. Several methods have been used to improve the detection of proteins on membranes, including glutaraldehyde treatment of nitrocellulose blots, elimination of keratin artifacts in immunoblots probed with polyclonal antibodies, and the washing of immunoblots with excessive water and manipulation of Tween-20 in wash buffer. These methods are briefly reviewed in this chapter.

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Authors and Affiliations

  1. Oklahoma Medical Research Foundation, 825 NE 13th Street, OK 73104, Oklahoma City, USA

    Biji T. Kurien & R. Hal Scofield

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  1. Biji T. Kurien
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  2. R. Hal Scofield
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Correspondence to Biji T. Kurien .

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Editors and Affiliations

  1. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    R. Hal Scofield

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Kurien, B.T., Scofield, R.H. (2009). A Brief Review of Other Notable Protein Detection Methods on Blots. In: Kurien, B., Scofield, R. (eds) Protein Blotting and Detection. Methods in Molecular Biology, vol 536. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-542-8_56

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  • DOI: https://doi.org/10.1007/978-1-59745-542-8_56

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-73-2

  • Online ISBN: 978-1-59745-542-8

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