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Microarrays in Blood Group Genotyping

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Molecular Typing of Blood Cell Antigens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1310))

Abstract

Thirty-five blood group systems, containing more than 300 antigens, are listed by the International Society of Blood Transfusion (ISBT). Most of these antigens result from a single-nucleotide polymorphism (SNP). Blood group typing is conventionally carried out by serology. However, this technique has certain limitations and cannot respond to the growing demand for blood products typed for a large number of antigens. Here we describe a blood group genotyping assay, from genomic DNA extraction from whole-blood samples to results. After DNA extraction, the on-chip test is based on the hybridization of targets beforehand amplified by multiplex polymerase chain reaction, followed by a revelation step allowing the simultaneous identification of up to 24 blood group antigens and leading to the determination of extended genotypes.

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References

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Author information

Authors and Affiliations

  1. AXO Science SAS, Villeurbanne, France

    Stephanie A. Boccoz & Gaëlle Le Goff

  2. Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Equipe Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires (GEMBAS)UMR 5246 ICBMS, Université Lyon 1 - CNRS, Villeurbanne, France

    Loïc J. Blum

  3. Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Equipe Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires (GEMBAS)UMR 5246 ICBMS, Université Lyon 1 - CNRS, Villeurbanne, Cedex, 69622, France

    Christophe A. Marquette

Authors
  1. Stephanie A. Boccoz
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  2. Gaëlle Le Goff
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  3. Loïc J. Blum
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  4. Christophe A. Marquette
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Corresponding author

Correspondence to Christophe A. Marquette .

Editor information

Editors and Affiliations

  1. Medical Faculty Mannheim, German Red Cross Blood Service Baden-Wurttemberg – Hessen, Institute of Transfusion Medicine and Immunology, Heidelberg University, Mannheim, Germany

    Peter Bugert

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© 2015 Springer Science+Business Media New York

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Boccoz, S.A., Le Goff, G., Blum, L.J., Marquette, C.A. (2015). Microarrays in Blood Group Genotyping. In: Bugert, P. (eds) Molecular Typing of Blood Cell Antigens. Methods in Molecular Biology, vol 1310. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2690-9_9

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  • DOI: https://doi.org/10.1007/978-1-4939-2690-9_9

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2689-3

  • Online ISBN: 978-1-4939-2690-9

  • eBook Packages: Springer Protocols

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