Enzyme-Enhanced Extraction of Antioxidant Ingredients from Algae

  • Björn V. Adalbjörnsson
  • Rósa Jónsdóttir
Part of the Methods in Molecular Biology book series (MIMB, volume 1308)


Marine algae are not only a rich source of dietary fibre, proteins, vitamins, and minerals, but also contain a great variety of secondary metabolites with diverse biological activities. Marine macroalgae are a rich source of various natural antioxidants such as polyphenols, especially phlorotannins (made of polyphloroglucinol units) derived from brown algae, which play an important role in preventing lipid peroxidation. In recent years, a number of potent antioxidant compounds have been isolated and identified from different types of edible seaweeds. Extraction methods commonly used for the isolation of antioxidants are based on conventional water or organic solvent extractions. However, recent advances have shown that enzymatic hydrolysis can achieve higher yield of bioactive compounds from algae. Here we describe a method based on enzymatic hydrolysis which both increases yield and decreases cost associated with organic solvents. This method achieves cell wall disruption and breakdown of internal storage components for more effective release of intracellular bioactive compounds. In addition, hydrolysis of proteins produces peptides which may have antioxidant properties, thus enhancing the bioactivity of the algal extract. The method described can be used for production of extracts from red and brown macroalgal species.

Key words

Antioxidants Enzyme hydrolysis Extraction Glycosidases Macroalgae Proteases 


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Copyright information

© Springer Science+Business Media, New York 2015

Authors and Affiliations

  1. 1.Biotechnology and Biomolecules/Faculty of Food Science and NutritionMatis Ltd. Icelandic Food and Biotech R&D/University of IcelandReykjavíkIceland
  2. 2.Biotechnology and BiomoleculesMatis Ltd. Icelandic Food and Biotech R&DReykjavíkIceland

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