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Phosphatases in Plants

  • Protocol
Plant Phosphoproteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1306))

Abstract

Reversible protein phosphorylation is an essential posttranslational modification mechanism executed by opposing actions of protein phosphatases and protein kinases. About 1,000 predicted kinases in Arabidopsis thaliana kinome predominate the number of protein phosphatases, of which there are only ~150 members in Arabidopsis. Protein phosphatases were often referred to as “housekeeping” enzymes, which act to keep eukaryotic systems in balance by counteracting the activity of protein kinases. However, recent investigations reveal the crucial and specific regulatory functions of phosphatases in cell signaling. Phosphatases operate in a coordinated manner with the protein kinases, to execute their important function in determining the cellular response to a physiological stimulus. Closer examination has established high specificity of phosphatases in substrate recognition and important roles in plant signaling pathways, such as pathogen defense and stress regulation, light and hormonal signaling, cell cycle and differentiation, metabolism, and plant growth. In this minireview we provide a compact overview about Arabidopsis protein phosphatase families, as well as members of phosphoglucan and lipid phosphatases, and highlight the recent discoveries in phosphatase research.

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Acknowledgements

We apologize to the readers for not covering numerous important studies and to authors, whose studies could not be mentioned in this minireview due to space limitations.

This work has received funding from the Lithuanian-Swiss cooperation program to reduce economic and social disparities within the enlarged European Union under project agreement No. CH-3-ŠMM-01/10, from the Lithuanian Research Council MIP-003/2014 and from the Austrian Science Fund (FWF) I255.

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  1. Institute of Biotechnology, University of Vilnius, V. Graičiūno 8, 02241, Vilnius, Lithuania

    Alois Schweighofer & Irute Meskiene

  2. Max F. Perutz Laboratories, University and Medical University of Vienna, Dr. Bohrgasse 9, 1030, Vienna, Austria

    Alois Schweighofer & Irute Meskiene

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  1. Alois Schweighofer
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  2. Irute Meskiene
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Correspondence to Alois Schweighofer .

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  1. Department of Plant Systems Biology, Universität Hohenheim, Stuttgart, Germany

    Waltraud X. Schulze

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Schweighofer, A., Meskiene, I. (2015). Phosphatases in Plants. In: Schulze, W. (eds) Plant Phosphoproteomics. Methods in Molecular Biology, vol 1306. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2648-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-2648-0_2

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