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Plant Phosphoproteomics

Methods and Protocols

  • Waltraud X. Schulze

Part of the Methods in Molecular Biology book series (MIMB, volume 1306)

Table of contents

  1. Front Matter
    Pages i-x
  2. Monika Zulawski, Waltraud X. Schulze
    Pages 1-23
  3. Alois Schweighofer, Irute Meskiene
    Pages 25-46
  4. Pingfang Yang
    Pages 47-57
  5. Xu Na Wu, Waltraud X. Schulze
    Pages 71-79
  6. Martin Thomas, Nicola Huck, Wolfgang Hoehenwarter, Uwe Conrath, Gerold J. M. Beckers
    Pages 81-96
  7. Xu Na Wu, Waltraud X. Schulze
    Pages 97-104
  8. Björn Ingelsson, Rikard Fristedt, Maria V. Turkina
    Pages 121-134
  9. Shisong Ma, Savithramma P. Dinesh-Kumar
    Pages 159-165
  10. Kristin Mayer, Sally Albrecht, Andreas Schaller
    Pages 167-176
  11. Han Cheng, Yongbo Wang, Zexian Liu, Yu Xue
    Pages 195-205
  12. Waltraud X. Schulze, Qiuming Yao, Dong Xu
    Pages 207-216
  13. Qiuming Yao, Waltraud X. Schulze, Dong Xu
    Pages 217-228
  14. Back Matter
    Pages 229-231

About this book

Introduction

This detailed volume addresses recent developments in phosphoproteomic techniques with a particular focus on the plant system. Over the recent decades, proteomic methods were refined to study the significance and dynamics of protein phosphorylation in various biological contexts. However, working with plant tissue imposes particular challenges to the biologist which are attributed to the rigid cell wall making protein extraction more difficult, the skewed protein abundance with Rubisco as a highly abundant protein, and a large central vacuole leading to low protein yield and increased degradative enzyme activity. The methodologies in this book seek to move beyond these issues.  Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Plant Phosphoproteomics: Methods and Protocols serves as an ideal reference for researchers investigating this vital area of plant science.

Keywords

Kinases Phosphoproteomic techniques Phosphorylation Plant system Plant tissue Posttranslational modifications Protein phosphorylation

Editors and affiliations

  • Waltraud X. Schulze
    • 1
  1. 1.Department of Plant Systems BiologyUniversität HohenheimStuttgartGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2648-0
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2647-3
  • Online ISBN 978-1-4939-2648-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site