Abstract
High-throughput imaging of yeast cells expressing fluorescent proteins can be used to understand biological pathways in the context of spatial organization. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.
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Acknowledgments
We thank David Gallo and Jason Hendry for critical reading of the manuscript, Johnny Tkach for establishing our original imaging procedure, and the Boone, Andrews, and Moffat labs for sharing their high content screening expertise. This work was supported by Canadian Cancer Society grant number 702310.
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Torres, N.P., Brown, G.W. (2015). A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells. In: Vengrova, S., Dalgaard, J. (eds) DNA Replication. Methods in Molecular Biology, vol 1300. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2596-4_1
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DOI: https://doi.org/10.1007/978-1-4939-2596-4_1
Publisher Name: Humana Press, New York, NY
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