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Assays for Induction of the Unfolded Protein Response and Selective Activation of the Three Major Pathways

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Stress Responses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1292))

Abstract

The endoplasmic reticulum (ER) is responsible for the proper folding and processing of secreted and transmembrane proteins within the cell. Stimuli that disrupt ER function cause an accumulation of misfolded proteins within the ER lumen, a condition termed ER stress. The unfolded protein response (UPR) is activated in response to ER stress in an attempt to restore ER homeostasis. UPR is initiated by three transmembrane sensors that activate three signaling pathways which lead to the activation of transcription factors and production of chaperones. The coordinated action of these three pathways attempt to restore homeostasis. However, if the ER homeostasis cannot be restored, it initiates apoptosis. Deregulated or compromised functions of these pathways can therefore lead to the pathogenesis of disease. In order to understand the molecular mechanisms involved, it is important to study each pathway independently. Here, we describe a number of approaches to selectively target each arm of UPR and investigate the functional significance of the UPR pathway involved.

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Acknowledgments

This publication has emanated from research conducted with the financial support of Health Research Board (grant numbers HRA_HSR/2010/24) to S.G.

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    Authors and Affiliations

    1. Discipline of Pathology, School of Medicine, National University of Ireland, Galway, Ireland

      Ananya Gupta & Danielle E. Read

    2. Discipline of Pathology, School of Medicine, Clinical Science Institute, National University of Ireland, Costello Road, Galway, Ireland

      Sanjeev Gupta Ph.D.

    Authors
    1. Ananya Gupta
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    2. Danielle E. Read
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    3. Sanjeev Gupta Ph.D.
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    Correspondence to Sanjeev Gupta Ph.D. .

    Editor information

    Editors and Affiliations

    1. Wentworth Institute of Technology, Boston, Massachusetts, USA

      Christine M. Oslowski

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    Gupta, A., Read, D.E., Gupta, S. (2015). Assays for Induction of the Unfolded Protein Response and Selective Activation of the Three Major Pathways. In: Oslowski, C. (eds) Stress Responses. Methods in Molecular Biology, vol 1292. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2522-3_2

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    • DOI: https://doi.org/10.1007/978-1-4939-2522-3_2

    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-2521-6

    • Online ISBN: 978-1-4939-2522-3

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