Abstract
Separating cells from a heterogeneous sample on microfluidic devices is a very important unit operation in biological and medical research. Microfluidic affinity cell chromatography is a label-free separation technique, providing ease of operation, low cost, and rapid analysis. In this chapter, protocols for cell affinity separation in polydimethylsiloxane (PDMS)–glass microdevices and glass capillaries are described.
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References
Pappas D, Wang K (2007) Cellular separations: a review of new challenges in analytical chemistry. Anal Chim Acta 601:26–35
Jiao LR, Apostolopoulos C, Jacob J, Szydlo R, Johnson N, Tsim N, Habib NA, Coombes RC, Stebbing J (2009) Unique localization of circulating tumor cells in patients with hepatic metastases. J Clin Oncol 27:6160–6165
Arora A, Simone G, Salieb-Beugelaar GB, Kim JT, Manz A (2010) Latest developments in micro total analysis systems. Anal Chem 82:4830–4847
El-Ali J, Sorger PK, Jensen KF (2006) Cells on chips. Nature 442:403–411
Gao Y, Li W, Pappas D (2013) Recent advances in microfluidic cell separations. Analyst 138:4714–4721
Kim SM, Lee SH, Suh KY (2008) Cell research with physically modified microfluidic channels: a review. Lab Chip 8:1015–1023
Baret JC, Miller OJ, Taly V, Ryckelynck M, El-Harrak A, Frenz L, Rick C, Samuels ML, Hutchison JB, Agresti JJ, Link DR, Weitz DA, Griffiths AD (2009) Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity. Lab Chip 9:1850–1858
Saliba AE, Saias L, Psychari E, Minc N, Simon D, Bidard FC, Mathiot C, Pierga JY, Fraisier V, Salamero J, Saada V, Farace F, Vielh P, Malaquin L, Viovy JL (2010) Microfluidic sorting and multimodal typing of cancer cells in self-assembled magnetic arrays. Proc Natl Acad Sci U S A 107:14524–14529
Li P, Gao Y, Pappas D (2011) Negative enrichment of target cells by microfluidic affinity chromatography. Anal Chem 83:7863–7869
Li P, Gao Y, Pappas D (2012) Multiparameter cell affinity chromatography: separation and analysis in a single microfluidic channel. Anal Chem 84:8140–8148
Pappas D (2010) Cell-affinity chromatography. In: Pappas D (ed) Practical cell analysis. Wiley, New York, pp 137–142
Jing R, Bolshakov VI, Flavell AJ (2007) The Tagged Microarray Marker (TAM) method for high throughput detection of single nucleotide and indel polymorphisms. Nat Protoc 2:168–177
Wang K, Cometti B, Pappas D (2007) Isolation and counting of multiple cell types using an affinity separation device. Anal Chim Acta 601:1–9
Wang K, Marshall MK, Garza G, Pappas D (2008) Open-tubular capillary cell affinity chromatography: single and tandem blood cell separation. Anal Chem 80:2118–2124
Acknowledgements
The authors acknowledge support from the National Institutes of Health (RR025782 and GM103550).
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Gao, Y., Li, W., Zhang, Y., Pappas, D. (2015). Cell Affinity Separations on Microfluidic Devices. In: Reichelt, S. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 1286. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2447-9_6
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DOI: https://doi.org/10.1007/978-1-4939-2447-9_6
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2446-2
Online ISBN: 978-1-4939-2447-9
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