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Cell Affinity Separations on Microfluidic Devices

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Affinity Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1286))

Abstract

Separating cells from a heterogeneous sample on microfluidic devices is a very important unit operation in biological and medical research. Microfluidic affinity cell chromatography is a label-free separation technique, providing ease of operation, low cost, and rapid analysis. In this chapter, protocols for cell affinity separation in polydimethylsiloxane (PDMS)–glass microdevices and glass capillaries are described.

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Acknowledgements

The authors acknowledge support from the National Institutes of Health (RR025782 and GM103550).

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Correspondence to Dimitri Pappas .

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© 2015 Springer Science+Business Media New York

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Gao, Y., Li, W., Zhang, Y., Pappas, D. (2015). Cell Affinity Separations on Microfluidic Devices. In: Reichelt, S. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 1286. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2447-9_6

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  • DOI: https://doi.org/10.1007/978-1-4939-2447-9_6

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2446-2

  • Online ISBN: 978-1-4939-2447-9

  • eBook Packages: Springer Protocols

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