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A β-Lactamase Based Assay to Measure Surface Expression of Membrane Proteins

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Membrane Trafficking

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1270))

Abstract

Measurement of cell surface expression is an essential part of studying membrane proteins. Traditional techniques for measuring surface expression depend on the availability of appropriate radioligands or antibodies towards extracellular epitopes of a protein of interest. The current protocol outlines the use of an assay to monitor surface expression of membrane proteins tagged with a bacterial β-lactamase in mammalian cell lines. The use of this technique allows for quick, quantitative, sensitive, and inexpensive measurement of surface expression, with the potential for high-throughput screening.

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References

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Acknowledgement

This work was supported by CIHR and NSERC operating grants to A.S. V.M.L. was partially funded by an OGS graduate fellowship. P.B. was partially funded by OGS and CIHR graduate fellowships.

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Authors and Affiliations

  1. Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada

    Pieter Beerepoot & Vincent M. Lam

  2. Department of Pharmacology and Toxicology, University of Toronto, Room 4302, Medical Sciences Building, 1 King’s College Circle, Toronto, ON, Canada, M5S 1A8

    Ali Salahpour

Authors
  1. Pieter Beerepoot
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  2. Vincent M. Lam
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  3. Ali Salahpour
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Corresponding author

Correspondence to Ali Salahpour .

Editor information

Editors and Affiliations

  1. Department of Biochemistry, National University of Singapore, Singapore, Singapore

    Bor Luen Tang

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© 2015 Springer Science+Business Media New York

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Beerepoot, P., Lam, V.M., Salahpour, A. (2015). A β-Lactamase Based Assay to Measure Surface Expression of Membrane Proteins. In: Tang, B. (eds) Membrane Trafficking. Methods in Molecular Biology, vol 1270. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2309-0_8

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  • DOI: https://doi.org/10.1007/978-1-4939-2309-0_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2308-3

  • Online ISBN: 978-1-4939-2309-0

  • eBook Packages: Springer Protocols

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