Chemical Genetic Screens Using Arabidopsis thaliana Seedlings Grown on Solid Medium
Genetic screening has been a powerful tool in identifying new genes in a pathway of interest (forward genetics) or attributing function to a particular gene via mutagenesis (reverse genetics). Small molecule-based chemical genetics is increasingly adapted in Arabidopsis research as a tool for similar purposes, i.e., to identify genes involved in certain biological processes and to dissect the biological roles of a gene. Chemical genetic screens have been successful in circumventing genetic redundancy to assign biological roles to a gene family as well as novel functions for well-known genes. Here, we describe how to screen Arabidopsis seedlings grown on solid medium with chemical compounds.
Key wordsChemical genetics Small molecules Genetic screens Arabidopsis seedlings Solid medium
Research in the Chen lab is supported by funds from Howard Hughes Medical Institute, Gordon and Betty Moore Foundation (GBMF3046), National Institutes of Health (GM061146), and National Institutes of Food and Agriculture (2010-04209). T.T.D. was supported by the National Science Foundation ChemGen IGERT program (DGE0504249) and NIH NIAID, Molecular and Cellular Immunobiology (5T32 AI07290) fellowship.
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