Abstract
Biocatalysis and biotransformations have a broad application in industrial synthetic chemistry. In addition to the whole cell catalysis, purified recombinant enzymes are successfully used for biocatalysis of specific chemical reactions. In this contribution, we report characterization, immobilization, and application of several model target enzymes (d-amino acid oxidase, sialic acid aldolase, maltodextrin phosphorylase, polyphosphate kinase) physiologically aggregated within inclusion bodies (IBs) retaining their biological activity as immobilized biocatalysts.
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Acknowledgments
This work was supported by the VEGA project No.: 2/0136/13. This contribution is the result of implementation and realization of the project ITMS-26220120054 supported by the Research and Development Operational Programme funded by the ERDF.
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Hrabárová, E., Achbergerová, L., Nahálka, J. (2015). Insoluble Protein Applications: The Use of Bacterial Inclusion Bodies as Biocatalysts. In: García-Fruitós, E. (eds) Insoluble Proteins. Methods in Molecular Biology, vol 1258. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2205-5_24
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DOI: https://doi.org/10.1007/978-1-4939-2205-5_24
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