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Dissecting a Model of Depressive-Related Phenotype and Antidepressant Effects in 129S2/SvPas Mice

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Serotonin Receptor Technologies

Part of the book series: Neuromethods ((NM,volume 95))

Abstract

As depressive disorder becomes one of the most prevalent neuropsychiatric dysfunctions worldwide, good animal models for studying depression and uncovering new targets for antidepressants are required. Since genetic factors clearly contribute to the manifestation of depression, transgenic mouse models offer seminal tools to disentangle this complex disorder. The behavioral and neurochemical responses of transgenic mice are the result of both the targeted or affected gene itself and its interactions with endogenous factors which, depending on the strain’s genotype, may differ significantly. A well-established strain to generate transgenic mice is the 129S2/SvPas strain. However, once a transgenic strain has been established, the animals are frequently backcrossed to C57BL/6 mice. As a consequence, experimental conditions for most paradigms are well established for C57BL/6 mice, but they might not be adequately adapted for other mouse models. Here, we are describing steps to establish a model of depressive-like state adapted to 129S2/SvPas mice. By setting the experimental conditions appropriate for 129S2/SvPas mice, costly and time-consuming backcrossing could be prevented and, most importantly, a more ethical use of laboratory animals is promoted.

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Acknowledgments

We thank Drs. C. Mombereau and S. Fernández for invaluable critical advises along the development of this project, N. Narboux-Nême for his collaboration with animal work, and DVM. Begoña Peñalba for sharing her expertise in submandibular bleeding technique. This work was supported by the Centre National de la Recherche Scientifique, the Institut National de la Santé et de la Recherche Médicale, the Université Pierre et Marie Curie, and grants from the Fondation de France, and the French Ministry of Research (Agence Nationale pour la Recherche ANR-12-BSV1-0015-01). S. Diaz was supported by fellowships from IBRO and from Region Ile de France DIM STEM. LM’s team is part of the École des Neurosciences de Paris Ile-de-France network and of the Bio-Psy Labex.

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Authors and Affiliations

  1. INSERM, UMR-S839, F-75005, Paris, France

    Silvina L. Diaz & Luc Maroteaux

  2. Sorbonne Universités, UPMC Univ Paris 06, UMR-S 839, F-75005, Paris, France

    Silvina L. Diaz & Luc Maroteaux

  3. Institut du Fer à Moulin, F-75005, Paris, France

    Silvina L. Diaz & Luc Maroteaux

  4. Instituto de Biología Celular y Neurociencia “Prof. Eduardo De Robertis”, CONICET – UBA, Paraguay 2155, 3 piso, C1121ABG, Buenos Aires, Argentina

    Silvina L. Diaz

  5. Luc Maroteaux INSERM, U839, Institut du Fer à Moulin, 17 rue du Fer à Moulin, 75005, Paris, France

    Luc Maroteaux

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  1. Silvina L. Diaz
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  2. Luc Maroteaux
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Correspondence to Luc Maroteaux .

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Editors and Affiliations

  1. Universität zu Köln, Köln, Germany

    Wolfgang Blenau

  2. Forschungszentrum Jülich, Jülich, Germany

    Arnd Baumann

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Diaz, S.L., Maroteaux, L. (2015). Dissecting a Model of Depressive-Related Phenotype and Antidepressant Effects in 129S2/SvPas Mice. In: Blenau, W., Baumann, A. (eds) Serotonin Receptor Technologies. Neuromethods, vol 95. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2187-4_4

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  • DOI: https://doi.org/10.1007/978-1-4939-2187-4_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2186-7

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