Book Volume 95 2015

Serotonin Receptor Technologies

Editors:

ISBN: 978-1-4939-2186-7 (Print) 978-1-4939-2187-4 (Online)

Table of contents (12 protocols)

  1. Front Matter

    Pages i-xii

  2. Receptor Dimerization

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-20

      Novel Approaches to Serotonin Receptor Interaction Studies

    3. No Access

      Protocol

      Pages 21-36

      Techniques for the Study of GPCR Heteromerization in Living Cells and Animal Models

  3. Manipulation and Analysis of Serotonergic Signaling in Brain Slices

    1. Front Matter

      Pages 37-37

    2. No Access

      Protocol

      Pages 39-56

      Organotypic Slices and Biolistic Transfection for the Study of Serotonin Receptor Function in CNS Neurons

  4. Mouse Models to Study Serotonin-Associated Behavioral Disorders

    1. Front Matter

      Pages 57-57

    2. No Access

      Protocol

      Pages 59-82

      Dissecting a Model of Depressive-Related Phenotype and Antidepressant Effects in 129S2/SvPas Mice

    3. No Access

      Protocol

      Pages 83-100

      The Murine Serotonin Syndrome and the 5-HT1A Receptor: Behavioral Effects and Hypothermia

    4. No Access

      Protocol

      Pages 101-121

      5-HT4 Receptor Subtype, β-Arrestin Level, and Rapid-Onset Effects of Antidepressant Drugs

  5. Serotonin Transporter

    1. Front Matter

      Pages 123-123

    2. No Access

      Protocol

      Pages 125-149

      Autoshaping Memory Formation and Retention Loss: Are Serotonin and Other Neurotransmitter Transporters Involved?

    3. No Access

      Protocol

      Pages 151-167

      Flow Cytometry to Determine Serotonin Transporter Function in Human Peripheral Blood Cells

  6. Invertebrate Model Systems

    1. Front Matter

      Pages 169-169

    2. No Access

      Protocol

      Pages 171-189

      Functional Genomics of Serotonin Receptors in Helminth Parasites: Elucidation of Receptor Function Through RNA Interference (RNAi)

    3. No Access

      Protocol

      Pages 191-203

      Visualization of the Serotonin System in Drosophila Brain: Immunofluorescence and Confocal Microscopy

    4. No Access

      Protocol

      Pages 205-222

      Serotonin Modulates Adult Neurogenesis in an Invertebrate Model: Approaches to Receptor Localization and Function

    5. No Access

      Protocol

      Pages 223-239

      Immunolocalization of Serotonergic Neurons in Arthropod Developmental and Phylogenetic Neuroanatomy

  7. Back Matter

    Pages 241-245