Abstract
During central nervous system (CNS ) development, a large number of neurons die by apoptosis and are efficiently removed through phagocytosis . Since apoptosis and apoptotic cell clearance are highly conserved in evolution , relatively simple and easily accessible Drosophila embryonic CNS provides a good model to study molecular and cellular mechanisms of these processes. Here, we describe how to assess neuronal apoptosis and glial phagocytosis of apoptotic neurons using immunohistochemistry of whole fixed embryos and live imaging of developing embryonic CNS. Combination of these different strategies allows a comprehensive analysis of neuronal cell death in vivo .
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Acknowledgments
The work was supported by the Israel Science Foundation (grant no. 427/11).
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Shklyar, B., Levy-Adam, F., Kurant, E. (2015). Drosophila Model for Studying Phagocytosis Following Neuronal Cell Death . In: Lossi, L., Merighi, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 1254. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2152-2_26
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DOI: https://doi.org/10.1007/978-1-4939-2152-2_26
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Publisher Name: Humana Press, New York, NY
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