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Neuronal Cell Death

Methods and Protocols

  • Laura Lossi
  • Adalberto Merighi

Part of the Methods in Molecular Biology book series (MIMB, volume 1254)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Laura Lossi, Claudia Castagna, Adalberto Merighi
    Pages 1-18
  3. Molecular, Structural, Functional, and Genomic Changes in Dying Neurons

    1. Front Matter
      Pages 19-19
    2. Fernando C. Baltanás, Jorge Valero, Jose Ramón Alonso, Maria Teresa Berciano, Miguel Lafarga
      Pages 43-54
    3. María Calvo, Carlos Villalobos, Lucía Núñez
      Pages 73-85
    4. Silvia Alasia, Carolina Cocito, Adalberto Merighi, Laura Lossi
      Pages 99-113
    5. Matthew E. Pamenter, Gabriel G. Haddad
      Pages 153-163
  4. Cell Death in Neuropathology and Experimental Neuropathology

    1. Front Matter
      Pages 165-165
    2. Stan Krajewski, Jeffrey Wang, Tashmia Khan, Jonathan Liu, Chia-Hung Sze, Maryla Krajewska
      Pages 181-196
    3. Carla I. Tasca, Tharine Dal-Cim, Helena Cimarosti
      Pages 197-210
    4. Nicholas Hyun, Linda Z. Shi, Michael W. Berns
      Pages 211-226
    5. Eduardo M. Normando, Mohammad H. Dehabadi, Li Guo, Lisa A. Turner, Gaia Pollorsi, M. Francesca Cordeiro
      Pages 227-237
    6. Huajun Jin, Arthi Kanthasamy, Dilshan S. Harischandra, Vellareddy Anantharam, Ajay Rana, Anumantha Kanthasamy
      Pages 239-252
  5. Neural Stem Cells, Progenitors, and Gene Therapy Strategies

    1. Front Matter
      Pages 253-253
    2. Raj R. Rao, Shilpa Iyer
      Pages 255-267
    3. Peggy Marconi, Cornel Fraefel, Alberto L. Epstein
      Pages 269-293
    4. Cornel Fraefel, Peggy Marconi, Alberto L. Epstein
      Pages 295-316
    5. David Díaz, José Ramón Alonso, Eduardo Weruaga
      Pages 317-325
  6. Neuronal Death in Nonmammalian Models

    1. Front Matter
      Pages 327-327
    2. Alexander Hruscha, Bettina Schmid
      Pages 341-350
    3. Boris Shklyar, Flonia Levy-Adam, Estee Kurant
      Pages 359-368
  7. Back Matter
    Pages 369-396

About this book

Introduction

This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology.

Keywords

DNA synthesis/alterations RNA silencing calcium concentration cell viability cell volume dying neurons epifluorescence and digital holography manipulate neuronal stem cells and progenitors mitochondrial function neuropathology

Editors and affiliations

  • Laura Lossi
    • 1
  • Adalberto Merighi
    • 2
  1. 1.Dept. of Veterinary SciencesUniversity of TorinoGrugliascoItaly
  2. 2.Dept. of Veterinary SciencesUniversity of TorinoGrugliascoItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2152-2
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2151-5
  • Online ISBN 978-1-4939-2152-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site