Abstract
Following neuronal cell death at the cellular level and over several time points is challenging in living animal because of the difficulty of accessing and identifying individual neurons . In the eye of a living Drosophila , it is possible to visualize neurons thanks to the cornea neutraliz ation technique. This technique can be coupled to the generation of mosaic clones by the Tomato /GFP -FLP/FRT method to identify a group of photoreceptor neurons at a single-cell resolution . This method has proved to be efficient for the study of photoreceptor development and degeneration . In this chapter, I describe this method and focus on fatp mutant photoreceptor neuron degeneration.
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References
Ready DF, Hanson TE, Benzer S (1976) Development of the Drosophila retina, a neurocrystalline lattice. Dev Biol 53:217–240
Wolff T, Ready DF (1993) Pattern formation in the Drosophila retina. In: Bate M (ed) Development in Drosophila melanogaster. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 1277–1325
Franceschini N, Kirschfeld K (1971) In vivo optical study of photoreceptor elements in the compound eye of Drosophila. Kybernetik 8:1–13
Franceschini N, Kirschfeld K, Minke B (1981) Fluorescence of photoreceptor cells observed in vivo. Science 213:1264–1267
Pichaud F, Desplan C (2001) A new visualization approach for identifying mutations that affect differentiation and organization of the Drosophila ommatidia. Development 128:815–826
Mollereau B, Wernet MF, Beaufils P et al (2000) A green fluorescent protein enhancer trap screen in Drosophila photoreceptor cells. Mech Dev 93:151–160
Gambis A, Dourlen P, Steller H et al (2011) Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila. Dev Biol 351:128–134
Dourlen P, Bertin B, Chatelain G et al (2012) Drosophila fatty acid transport protein regulates rhodopsin-1 metabolism and is required for photoreceptor neuron survival. PLoS Genet 8:e1002833
Dourlen P, Levet C, Mejat A et al (2013) The Tomato/GFP-FLP/FRT method for live imaging of mosaic adult Drosophila photoreceptor cells. J Vis Exp: e50610
Golic KG (1991) Site-specific recombination between homologous chromosomes in Drosophila. Science 252:958–961
Franceschini N, Kirschfeld K (1971) Pseudopupil phenomena in the compound eye of drosophila. Kybernetik 9:159–182
Franceschini N (1972) Pupil and pseudopupil in the compound eye of Drosophila. In: Wehner R (ed) Information processing in the visual system of arthropods. Springer-Verlag, Berlin, pp 75–82
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Dourlen, P. (2015). In Vivo Assessment of Neuronal Cell Death in Drosophila . In: Lossi, L., Merighi, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 1254. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2152-2_25
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DOI: https://doi.org/10.1007/978-1-4939-2152-2_25
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