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Analysis of Receptor Tyrosine Kinase (RTK) Phosphorylation by Immunoblotting

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Receptor Tyrosine Kinases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1233))

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Abstract

Immunoblotting for phosphorylated forms of receptor tyrosine kinases (RTKs) has been the mainstay of investigations on RTK signaling for the past two decades. Despite the development of quantitative mass spectrometry, reverse-phase protein array, and multiplex technologies, immunoblotting with phospho-specific antibodies is still used in parallel with these technologies and remains a powerful, and reproducible, method for interrogating signaling networks involving RTKs.

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Acknowledgements

This work was supported by the Irish Research Council, Science Foundation Ireland (08SRCB410), the Health Research Board (CSA/2007/11), and the Cancer Clinical Research Trust.

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Authors and Affiliations

  1. Translational Cancer Therapeutics Program, Department of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC, USA

    Martina McDermott

  2. Molecular Therapeutics for Cancer Ireland, National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland

    Norma O’Donovan

Authors
  1. Martina McDermott
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  2. Norma O’Donovan
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Corresponding author

Correspondence to Norma O’Donovan .

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Editors and Affiliations

  1. Necker Hospital, Paris, France

    Serena Germano

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© 2015 Springer Science+Business Media New York

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McDermott, M., O’Donovan, N. (2015). Analysis of Receptor Tyrosine Kinase (RTK) Phosphorylation by Immunoblotting. In: Germano, S. (eds) Receptor Tyrosine Kinases. Methods in Molecular Biology, vol 1233. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1789-1_1

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  • DOI: https://doi.org/10.1007/978-1-4939-1789-1_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1788-4

  • Online ISBN: 978-1-4939-1789-1

  • eBook Packages: Springer Protocols

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