Abstract
Synthetic lipid bilayers have long been used as models of cell membranes. The compositional asymmetry in the eukaryotic plasma membrane is a key chemical characteristic of this membrane that has traditionally been difficult to reproduce in synthetic systems. In this chapter, we describe recent technologies for fabricating compositionally asymmetric giant unilamellar lipid vesicles (GUVs) and provide detailed protocols for a microfluidic-based fabrication technique.
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Acknowledgement
This work was supported by the National Institutes of Health under awards 1R21AG033890 and 1R01GM093279.
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Hu, P.C., Malmstadt, N. (2015). Asymmetric Giant Lipid Vesicle Fabrication. In: Owen, D. (eds) Methods in Membrane Lipids. Methods in Molecular Biology, vol 1232. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1752-5_7
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DOI: https://doi.org/10.1007/978-1-4939-1752-5_7
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