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Methods in Membrane Lipids

  • Dylan M. Owen

Part of the Methods in Molecular Biology book series (MIMB, volume 1232)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Richard M. Epand
    Pages 1-6
  3. Alexander P. Demchenko, Guy Duportail, Sule Oncul, Andrey S. Klymchenko, Yves Mély
    Pages 19-43
  4. Charles Cranfield, Sonia Carne, Boris Martinac, Bruce Cornell
    Pages 45-53
  5. Peichi C. Hu, Noah Malmstadt
    Pages 79-90
  6. Saleemulla Mahammad, Ingela Parmryd
    Pages 91-102
  7. G. W. Ashdown, Dylan M. Owen
    Pages 115-122
  8. Astrid Magenau, Katharina Gaus
    Pages 123-136
  9. Alison J. Beckett, Ian A. Prior
    Pages 137-151
  10. Charles Roduit, Giovanni Longo, Giovanni Dietler, Sandor Kasas
    Pages 153-159
  11. Eduard Hermann, Jonas Ries, Ana J. García-Sáez
    Pages 181-197
  12. Arwen I. I. Tyler, Robert V. Law, John M. Seddon
    Pages 199-225
  13. Arwen I. I. Tyler, James A. Clarke, John M. Seddon, Robert V. Law
    Pages 227-253
  14. Michael Carnell, Alex Macmillan, Renee Whan
    Pages 255-271
  15. Svetlana Baoukina, D. Peter Tieleman
    Pages 307-322
  16. Back Matter
    Pages 323-327

About this book

Introduction

This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and in silico.  Chapters focus on properties of the component lipids, membranes and their biophysical properties, fluorescent probes for studying membranes, sample preparation, physical techniques to study membrane composition, properties , and function, behavior of cholesterol within a bilayer and examination of cholesterol-dependent phase separation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative  and practical, Methods in Membrane Lipids, Second Edition seeks to aid scientist in further study into membrane lipids.

Keywords

Cell membranes X-Ray scattering archetypes artificial membranes asymmetric planar lipid bilayers atomic force microscopy cellular membranes component lipids fluorescence correlation spectroscopy fluorescent probes lipid phase behaviour nuclear magnetic resonance (NMR) photobleaching tethered lipid bilayers

Editors and affiliations

  • Dylan M. Owen
    • 1
  1. 1.Department of Physics and Randall Division of Cell and Molecular BiophysicsKing’s College LondonLondonUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-1752-5
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1751-8
  • Online ISBN 978-1-4939-1752-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site