Abstract
As the activation of opioid receptors leads to the modulation of potassium and calcium channels, the ion imaging represents an attractive method to analyze the function of the receptors. Here, we describe the imaging of potassium using the FluxOR™ potassium ion channel assay, and of calcium using Fura-2 acetoxymethyl ester. Specifically, we (1) characterize the activation of the G-protein-coupled inwardly rectifying potassium 2 channel by agonists of μ- and δ-opioid receptors with the aid of the FluxOR™ assay in cultured mouse dorsal root ganglion neurons, and (2) describe calcium imaging protocols to measure capsaicin-induced transient receptor potential vanilloid 1 channel activity during opioid withdrawal in transfected human embryonic kidney 293 cells.
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Acknowledgements
This work was supported by grants from the Deutsche Forschungsgemeinschaft (KFO 100/2), Bundesministerium für Bildung und Forschung (MedSys 0101-31P5783), and the European Society of Anesthesiology.
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Spahn, V., Nockemann, D., Machelska, H. (2015). Analysis of Potassium and Calcium Imaging to Assay the Function of Opioid Receptors. In: Spampinato, S. (eds) Opioid Receptors. Methods in Molecular Biology, vol 1230. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1708-2_15
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DOI: https://doi.org/10.1007/978-1-4939-1708-2_15
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1707-5
Online ISBN: 978-1-4939-1708-2
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