Editors:
- Includes cutting-edge methods and protocols in the study of opioid receptors
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1230)
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Table of contents (27 protocols)
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Front Matter
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Genetic, Structural Biology, Transcriptional and Post-transcriptional Analysis of Opioid Receptors
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Front Matter
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Cellular Detection and Analysis of Opioid Receptors
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Front Matter
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Analysis of Signaling Events Modulated by Opioid Receptors
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Front Matter
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About this book
Opioid Receptors: Methods and Protocols serves as a comprehensive guide to both key new techniques and established methods for the investigation of genetics, structural biology, transcription, and post-transcriptional events of opioid receptors. Other methods cover the cellular detection and trafficking of opioid receptors in vitro and in vivo. Procedures aimed to investigate signaling pathways modulated by opioid receptors and model systems to study opioid receptor-mediated functions are also included. Finally, methods to assay behavioral effects mediated by opioid receptors are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and authoritative, Opioid Receptors: Methods and Protocols will help both experienced and new entrants in this field to carry out their experiments successfully and with new inspiration.
Reviews
“Book edited by Santi M. Spampinato offers a review of state-of-the-art techniques used to analyze opioid receptors functionality on molecular, cellular, structural, and organism level. … The book is aimed for practicing researchers actively engaged in the field of opioid receptors investigation. Everyone from graduate students to principal investigator may find it useful.” (Lubica Lacinova, General Physiology and Biophysics, Vol. 34 (3), 2015)
Editors and Affiliations
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University of Bologna, Bologna, Italy
Santi M. Spampinato
Bibliographic Information
Book Title: Opioid Receptors
Book Subtitle: Methods and Protocols
Editors: Santi M. Spampinato
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1708-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-1707-5Published: 08 October 2014
Softcover ISBN: 978-1-4939-5550-3Published: 23 August 2016
eBook ISBN: 978-1-4939-1708-2Published: 07 October 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 326
Number of Illustrations: 30 b/w illustrations, 26 illustrations in colour
Topics: Pharmacology/Toxicology, Neurobiology