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Molecular Identification and Quantification of Human Rhinoviruses in Respiratory Samples

  • Wai-Ming LeeEmail author
  • Kris Grindle
  • Rose Vrtis
  • Tressa Pappas
  • Fue Vang
  • Iris Lee
  • James E. Gern
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1221)

Abstract

PCR-based molecular assays have become standard diagnostic procedures for the identification and quantification of human rhinoviruses (HRVs) and other respiratory pathogens in most, if not all, clinical microbiology laboratories. Molecular assays are significantly more sensitive than traditional culture-based and serological methods. This advantage has led to the recognition that HRV infections are common causes for not only upper airway symptoms but also more severe lower respiratory illnesses. In addition, molecular assays improve turnaround time, can be performed by technicians with ordinary skills, and can easily be automated. This chapter describes two highly sensitive and specific PCR-based methods for identifying and quantifying HRVs. The first is a two-step PCR method for the detection and typing of HRV. The second is a pan-HRV real-time quantitative (q) PCR method for measuring viral loads in respiratory samples.

Key words

Molecular typing HRV type Species Real-time quantitative PCR Viral load 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Wai-Ming Lee
    • 1
    Email author
  • Kris Grindle
    • 2
  • Rose Vrtis
    • 2
  • Tressa Pappas
    • 2
  • Fue Vang
    • 2
  • Iris Lee
    • 2
  • James E. Gern
    • 3
  1. 1.Biological Mimetics Inc.FrederickUSA
  2. 2.Department of Pediatrics, School of Medicine and Public HealthUniversity of WisconsinMadisonUSA
  3. 3.Department of Pediatrics and MedicineUniversity of Wisconsin School of Medicine and Public HealthMadisonUSA

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