Methods and Protocols

  • David A. Jans
  • Reena Ghildyal

Part of the Methods in Molecular Biology book series (MIMB, volume 1221)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Ann C. Palmenberg, James E. Gern
    Pages 1-10
  3. Wai-Ming Lee, Kris Grindle, Rose Vrtis, Tressa Pappas, Fue Vang, Iris Lee et al.
    Pages 25-38
  4. Wei Wang, Jing He, Yi Liu, Lei Xu, Wencai Guan, Yunwen Hu
    Pages 39-47
  5. Wai-Ming Lee, Yin Chen, Wensheng Wang, Anne Mosser
    Pages 49-61
  6. Shamaila Ashraf, Rebecca Brockman-Schneider, James E. Gern
    Pages 63-70
  7. Wai-Ming Lee, Yin Chen, Wensheng Wang, Anne Mosser
    Pages 71-81
  8. Shushan Harutyunyan, Arthur Sedivy, Gottfried Köhler, Heinrich Kowalski, Dieter Blaas
    Pages 83-100
  9. Victor U. Weiss, Xavier Subirats, Mohit Kumar, Shushan Harutyunyan, Irene Gösler, Heinrich Kowalski et al.
    Pages 101-128
  10. Lora M. Jensen, Erin J. Walker, David A. Jans, Reena Ghildyal
    Pages 129-141
  11. Lora M. Jensen, Erin J. Walker, Reena Ghildyal
    Pages 143-148
  12. Wai-Ming Lee, Wensheng Wang, Yury A. Bochkov, Iris Lee
    Pages 149-170
  13. Nathan W. Bartlett, Aran Singanayagam, Sebastian L. Johnston
    Pages 181-188
  14. Back Matter
    Pages 189-190

About this book


Rhinoviruses: Methods and Protocols highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as being more recently recognized as the major viral cause of asthma and chronic obstructive pulmonary disease (COPD) exacerbations. The huge advancements in technical applications of biophysics and improvements in high-end imaging techniques have also had implications for HRV research among other infectious diseases. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Rhinoviruses: Methods and Protocols seeks to serve both professionals and novices with the latest technical advances in HRV research to ultimately enable the development of therapeutic approaches to combat HRV, and most importantly, its pathogenic effects in asthma exacerbation.


high-end imaging techniques human rhinovirus human rhinovirus research molecular genotyping protease assays reverse genetics

Editors and affiliations

  • David A. Jans
    • 1
  • Reena Ghildyal
    • 2
  1. 1.Department of Biochemistry and Molecular Biology, Faculty of Health, Nursing and Medical Sciences,Monash UniversityMelbourneAustralia
  2. 2.Centre for Research in Therapeutic Solutions, Faculty of Education, Science, Technology and MathematicsUniversity of CanberraCanberraAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1570-5
  • Online ISBN 978-1-4939-1571-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site