Abstract
RNA silencing is a regulatory mechanism that controls the expression of endogenous genes and exogenous molecular parasites such as viruses, transgenes, and transposable elements. The sequence specificity of these processes relies on small noncoding RNA (sRNA) molecules. In plants, one of the most fascinating aspects of RNA silencing is its mobile nature, in other words its ability to spread from the cell where it has been initiated to neighboring cells, through movement of sRNA molecules. To study this process, a key step is to directly monitor the spread of these nucleic acid species. Here we describe how this can be achieved through biolistic delivery of fluorescently labeled siRNA.
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Acknowledgments
This work was supported by a research grant from the Agence Nationale pour la Recherche (ANR-10-LABX-36) and the Bettencourt-Schueller foundation.
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Himber, C., Dunoyer, P. (2015). The Tracking of Intercellular Small RNA Movement. In: Heinlein, M. (eds) Plasmodesmata. Methods in Molecular Biology, vol 1217. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1523-1_18
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DOI: https://doi.org/10.1007/978-1-4939-1523-1_18
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-1523-1
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