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Adipose-Derived Stem Cells: Methods for Isolation and Applications for Clinical Use

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Stem Cells and Tissue Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1210))

Abstract

Adipose tissue sciences have rapidly expanded since the identification of regenerative cells contained within the stromal vascular fraction (SVF) of fat. Isolation of the SVF, containing adipose-derived stem cells (ADSC), can be accomplished efficiently in the operating room or in the laboratory through enzymatic digestion of the adipose tissue and concentration of SVF. Cells can be directly re-injected as a mesotherapeutic agent, recombined with a tissue scaffold (e.g., cell-enriched fat grafts) or expanded in culture for tissue-engineered cell therapeutics. The potential for cell therapy is under current investigation by researchers around the world. This chapter reviews laboratory methods for isolating ADSCs and the ongoing clinical trials evaluating cell therapeutic efficacy across many specialties, including cardiology, neurology, immunology, tissue engineering, sports medicine, and plastic and reconstructive surgery.

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Acknowledgements

Research support is provided from the Plastic Surgery Educational Foundation, National Institutes of Health. Drs. Alfonso and Hicok are employees and Dr. Cohen is a consultant for Cytori Therapeutics. Dr. Cohen is a founder and board member of Cell Society.

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Authors and Affiliations

  1. Department of Surgery, University of California San Diego, San Diego, CA, USA

    Brian Mailey, Ava Hosseini, Jennifer Baker, Adam Young, Zeni Alfonso, Kevin Hicok, Anne M. Wallace & Steven R. Cohen

  2. Department of Bioengineering, University of California San Diego, San Diego, CA, USA

    Brian Mailey, Ava Hosseini, Jennifer Baker, Adam Young, Zeni Alfonso, Kevin Hicok, Anne M. Wallace & Steven R. Cohen

  3. Sanford Consortium for Regenerative Medicine, University of California San Diego, San Diego, CA, USA

    Brian Mailey, Ava Hosseini, Jennifer Baker, Adam Young, Zeni Alfonso, Kevin Hicok, Anne M. Wallace & Steven R. Cohen

  4. FACES+, 4510 Executive Center Drive, Suite #200, San Diego, CA, 92121, USA

    Brian Mailey, Ava Hosseini, Jennifer Baker, Adam Young, Zeni Alfonso, Kevin Hicok, Anne M. Wallace & Steven R. Cohen

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  1. Brian Mailey
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Correspondence to Brian Mailey .

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  1. Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, Oregon, USA

    Chrissa Kioussi

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Mailey, B. et al. (2014). Adipose-Derived Stem Cells: Methods for Isolation and Applications for Clinical Use. In: Kioussi, C. (eds) Stem Cells and Tissue Repair. Methods in Molecular Biology, vol 1210. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1435-7_13

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  • DOI: https://doi.org/10.1007/978-1-4939-1435-7_13

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  • Publisher Name: Humana Press, New York, NY

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  • Online ISBN: 978-1-4939-1435-7

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