Adipose-Derived Stem Cells: Methods for Isolation and Applications for Clinical Use

  • Brian MaileyEmail author
  • Ava Hosseini
  • Jennifer Baker
  • Adam Young
  • Zeni Alfonso
  • Kevin Hicok
  • Anne M. Wallace
  • Steven R. Cohen
Part of the Methods in Molecular Biology book series (MIMB, volume 1210)


Adipose tissue sciences have rapidly expanded since the identification of regenerative cells contained within the stromal vascular fraction (SVF) of fat. Isolation of the SVF, containing adipose-derived stem cells (ADSC), can be accomplished efficiently in the operating room or in the laboratory through enzymatic digestion of the adipose tissue and concentration of SVF. Cells can be directly re-injected as a mesotherapeutic agent, recombined with a tissue scaffold (e.g., cell-enriched fat grafts) or expanded in culture for tissue-engineered cell therapeutics. The potential for cell therapy is under current investigation by researchers around the world. This chapter reviews laboratory methods for isolating ADSCs and the ongoing clinical trials evaluating cell therapeutic efficacy across many specialties, including cardiology, neurology, immunology, tissue engineering, sports medicine, and plastic and reconstructive surgery.

Key words

Adipose-derived stem or regenerative cells Isolation Laboratory methods Clinical trials 



Research support is provided from the Plastic Surgery Educational Foundation, National Institutes of Health. Drs. Alfonso and Hicok are employees and Dr. Cohen is a consultant for Cytori Therapeutics. Dr. Cohen is a founder and board member of Cell Society.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Brian Mailey
    • 1
    • 2
    • 3
    • 4
    Email author
  • Ava Hosseini
    • 1
    • 2
    • 3
    • 4
  • Jennifer Baker
    • 1
    • 2
    • 3
    • 4
  • Adam Young
    • 1
    • 2
    • 3
    • 4
  • Zeni Alfonso
    • 1
    • 2
    • 3
    • 4
  • Kevin Hicok
    • 1
    • 2
    • 3
    • 4
  • Anne M. Wallace
    • 1
    • 2
    • 3
    • 4
  • Steven R. Cohen
    • 1
    • 2
    • 3
    • 4
  1. 1.Department of SurgeryUniversity of California San DiegoSan DiegoUSA
  2. 2.Department of BioengineeringUniversity of California San DiegoSan DiegoUSA
  3. 3.Sanford Consortium for Regenerative MedicineUniversity of California San DiegoSan DiegoUSA
  4. 4.FACES+San DiegoUSA

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