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Chromatin Immunoprecipitation Protocol for Mammalian Cells

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Transcription Factor Regulatory Networks

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1164))

Abstract

The genomic approach (ChIP-seq) we introduce here is now a widely used powerful tool to explore protein–DNA interaction at genome-wide level in high resolution. This technology opens up the way to understand how local event mediated by protein–protein or protein–DNA interactions lead to the dynamic changes of overall chromosome structure and how variety of proteins make a regulatory network for the faithful execution of various chromosomal functions (i.e., transcription, replication, recombination, repair, and partition).

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References

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Acknowledgement

We are grateful to T. Sutani, H. Masuda, and C. Renard for discussions, help, and advice.

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Authors and Affiliations

  1. Laboratory of Genome Structure and Function, Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan

    Makiko Komata, Yuki Katou, Hiroshi Tanaka, Ryuichiro Nakato, Katsuhiko Shirahige & Masashige Bando

Authors
  1. Makiko Komata
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  2. Yuki Katou
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  3. Hiroshi Tanaka
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  4. Ryuichiro Nakato
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  5. Katsuhiko Shirahige
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  6. Masashige Bando
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Corresponding author

Correspondence to Masashige Bando .

Editor information

Editors and Affiliations

  1. Division of Interactome Medical Science, The University of Tokyo, The Institute of Medical Science, Minato-ku, Tokyo, Japan

    Etsuko Miyamoto-Sato

  2. Division of Interactome Medical Science, The University of Tokyo, The Institute of Medical Science, Minato-ku, Tokyo, Japan

    Hiroyuki Ohashi

  3. Division of Interactome Medical Science, The University of Tokyo, The Institute of Medical Science, Minato-ku, Tokyo, Japan

    Hirotaka Sasaki

  4. Division of Interactome Medical Science, The University of Tokyo, The Institute of Medical Science, Minato-ku, Tokyo, Japan

    Jun-ichi Nishikawa

  5. Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Kohoku-ku, Yokohama, Japan

    Hiroshi Yanagawa

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Komata, M., Katou, Y., Tanaka, H., Nakato, R., Shirahige, K., Bando, M. (2014). Chromatin Immunoprecipitation Protocol for Mammalian Cells. In: Miyamoto-Sato, E., Ohashi, H., Sasaki, H., Nishikawa, Ji., Yanagawa, H. (eds) Transcription Factor Regulatory Networks. Methods in Molecular Biology, vol 1164. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0805-9_4

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  • DOI: https://doi.org/10.1007/978-1-4939-0805-9_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0804-2

  • Online ISBN: 978-1-4939-0805-9

  • eBook Packages: Springer Protocols

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