Abstract
Stem cell in vitro culture is a useful model system to study mechanisms underlying transitions between defined cell states. Epiblast stem cells, in addition to being capable of somatic differentiation, can be converted to a more primitive embryonic stem cell-like state, by overexpression of specific transcription factors. Here, we describe a reliable method to accomplish—and potentially further study—the transgene-independent reversion from epiblast stem cells to ES cells using administration of specific growth factors and small molecule inhibitors.
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Acknowledgment
This work was supported by the Bundesinstitut für Risikobewertung (BfR) grant FK-3-1329-471 and the Chemical Genomics Centre of the Max Planck Society.
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Rao, J., Greber, B. (2014). Conversion of Epiblast Stem Cells to Embryonic Stem Cells Using Growth Factors and Small Molecule Inhibitors. In: Kidder, B. (eds) Stem Cell Transcriptional Networks. Methods in Molecular Biology, vol 1150. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0512-6_14
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DOI: https://doi.org/10.1007/978-1-4939-0512-6_14
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0511-9
Online ISBN: 978-1-4939-0512-6
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