Stem Cell Transcriptional Networks

Methods and Protocols

  • Benjamin L. Kidder

Part of the Methods in Molecular Biology book series (MIMB, volume 1150)

Table of contents

  1. Front Matter
    Pages i-x
  2. Next-Generation Sequencing Library Preparation and Data Analysis

  3. Visual Analysis and Interpretation of Large-Scale Interaction Networks

    1. Front Matter
      Pages 113-113
    2. Patrick S. Stumpf, Ben D. MacArthur
      Pages 131-139
    3. Tao Ye, Sarina Ravens, Arnaud R. Krebs, Làszlò Tora
      Pages 141-152
  4. Transcriptional Networks in Embryonic and Adult Stem Cell

  5. Embryo Culture and Derivation of Stem Cells

  6. Transcriptional Programs that Promote Self-Renewal, Reprogramming, and Transdifferentiation

About this book

Introduction

Stem Cell Transcriptional Networks: Methods and Protocols collects techniques used to increase our understanding of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. The volume opens with a section on next-generation sequencing library preparation and data analysis. Continuing with a collection of protocols on visual analysis and interpretation of large-scale interaction networks, this detailed compilation features transcriptional networks in embryonic and adult stem cells, embryo culture and derivation of stem cells, as well as transcriptional programs that promote self-renewal, reprogramming, and transdifferentiation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Stem Cell Transcriptional Networks: Methods and Protocols aims to provide a key resource for biologists seeking to interrogate these vital networks.

Keywords

Cellular expression Epigenetic landscapes Next-generation sequencing Protein-DNA binding interactions Regenerative medicine Stem cell biology Transcriptional programs

Editors and affiliations

  • Benjamin L. Kidder
    • 1
  1. 1.Systems Biology CenterNational Heart, Lung, and Blood Institute, National Institutes of HealthBethesdaUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-0512-6
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0511-9
  • Online ISBN 978-1-4939-0512-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book