Abstract
Quantitative real-time PCR (qPCR) is a mature technology that can be used to accurately quantify the number of copies of a target nucleic acid in a sample. Here, we describe a method for using this technology to determine the copy number of a transgene stably integrated into a plant’s genome and to ascertain the level of transgene expression.
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Fletcher, S.J. (2014). qPCR for Quantification of Transgene Expression and Determination of Transgene Copy Number. In: Fleury, D., Whitford, R. (eds) Crop Breeding. Methods in Molecular Biology, vol 1145. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0446-4_17
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DOI: https://doi.org/10.1007/978-1-4939-0446-4_17
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0445-7
Online ISBN: 978-1-4939-0446-4
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