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Purification of Rubisco from Leaves

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Photosynthesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2790))

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Abstract

Rubisco fixes CO2 through the carboxylation of ribulose 1,5-bisphosphate (RuBP) during photosynthesis, enabling the synthesis of organic compounds. The natural diversity of Rubisco properties represents an opportunity to improve its performance and there is considerable research effort focusing on better understanding the properties and regulation of the enzyme. This chapter describes a method for large-scale purification of Rubisco from leaves. After the extraction of Rubisco from plant leaves, the enzyme is separated from other proteins by fractional precipitation with polyethylene glycol followed by ion-exchange chromatography. This method enables the isolation of Rubisco in large quantities for a wide range of biochemical applications.

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Acknowledgments

The authors thank Abbie Whiteside for the preparation of Fig 2. The authors acknowledge funding through the European Union’s Horizon2020 research and innovation programme projects CAPITALISE (grant number 862201; JA, ECS) and PhotoBoost (grant number 862127; AKML, ECS).

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Author notes

  1. Authors Joana Amaral and Ana K. M. Lobo have equally contributed to this chapter.

Authors and Affiliations

  1. Lancaster Environment Centre, Lancaster University, Lancaster, UK

    Joana Amaral, Ana K. M. Lobo, Elizabete Carmo-Silva & Douglas J. Orr

Authors
  1. Joana Amaral
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  2. Ana K. M. Lobo
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  3. Elizabete Carmo-Silva
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  4. Douglas J. Orr
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Corresponding author

Correspondence to Douglas J. Orr .

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Editors and Affiliations

  1. Colorado Springs, CO, USA

    Sarah Covshoff

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Amaral, J., Lobo, A.K.M., Carmo-Silva, E., Orr, D.J. (2024). Purification of Rubisco from Leaves. In: Covshoff, S. (eds) Photosynthesis . Methods in Molecular Biology, vol 2790. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3790-6_22

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  • DOI: https://doi.org/10.1007/978-1-0716-3790-6_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3789-0

  • Online ISBN: 978-1-0716-3790-6

  • eBook Packages: Springer Protocols

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