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Photosynthesis

Methods and Protocols

  • Book
  • © 2024
  • Latest edition

Overview

Editors:
  1. Sarah Covshoff

    1. Colorado Springs, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2790)

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About this book

This fully updated edition explores fundamental protocols for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Featuring protocols to examine light response curves and gas exchange measurements for a variety of samples, the book includes new protocols on photosynthesis in the adaxial and abaxial sides of a leaf, non-foliar organs, and aquatic systems. Protocols and best practice for eddy covariance, thermal, spectral, and hyperspectral imaging and data analysis, as well as stable isotope labeling and quantification of photosynthetic metabolites, are introduced alongside fresh insights on many more topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Photosynthesis: Methods and Protocols, Second Edition provides an ideal guide to the improved technologies expanding our capabilities to study photosynthesis today.

Keywords

Table of contents (24 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Tools for Measuring Photosynthesis at Different Scales

    • Berkley J. Walker, Steven M. Driever, Johannes Kromdijk, Tracy Lawson, Florian A. Busch
    Pages 1-26

  3. Light Response Curves in Land Plants

    • Robert A. Coe, Hsiang-Chun Lin
    Pages 27-39

  4. Photosynthetic Gas Exchange in Land Plants at the Leaf Level

    • Florian A. Busch
    Pages 41-61

  5. Simultaneous and Independent Abaxial and Adaxial Gas Exchange Measurements

    • Shellie Wall, Pauline Lemonnier, Alexandra L. Milliken, Phillip Davey, Tracy Lawson
    Pages 63-76

  6. Measuring Nonfoliar Photosynthesis

    • Alexandra L. Milliken, Mengjie Fan, Jyotirmaya Mathan, Phillip Davey, Tracy Lawson
    Pages 77-94

  7. Measurements of Carbon Assimilation in Aquatic Systems

    • Phillip Davey, Tracy Lawson
    Pages 95-120

  8. Measurement of Algal Photosynthesis Using a Clark-Type O2 Electrode

    • Steven J. Burgess, Chandra Davies
    Pages 121-132

  9. Measurements of Oxygen Evolution in Photosynthesis

    • Dmitry Shevela, Wolfgang P. Schröder, Johannes Messinger
    Pages 133-148

  10. Measurement of O2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry

    • Steven M. Driever
    Pages 149-162

  11. Using Carbon Stable Isotopes to Study C3 and C4 Photosynthesis: Models and Calculations

    • Nerea Ubierna, Meisha-Marika Holloway-Phillips, Lisa Wingate, Jérôme Ogée, Florian A. Busch, Graham D. Farquhar
    Pages 163-211

  12. Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS)

    • Qingfeng Song, Xin-Guang Zhu
    Pages 213-226

  13. A Beginner’s Guide to Eddy Covariance: Methodology and Its Applications to Photosynthesis

    • Caitlin E. Moore, Anne Griebel
    Pages 227-256

  14. Chlorophyll Fluorescence on the Fast Timescale

    • Olubukola O. Ajigboye, Rumiana V. Ray, Erik H. Murchie
    Pages 257-267

  15. Shining Light into a “Black Box”: Essential Rationale Underlying Multiphase Flash Methodology

    • Thomas J. Avenson, Dayle K. McDermitt
    Pages 269-292

  16. Imaging Spatial and Temporal Variation in Photosynthesis Using Chlorophyll Fluorescence

    • Tracy Lawson, Silvere Vialet-Chabrand
    Pages 293-316

  17. Using Infrared Thermography for High-Throughput Plant Phenotyping

    • Mengjie Fan, John Stamford, Tracy Lawson
    Pages 317-332

  18. Spectral Reflectance Measurements

    • John Stamford, Piotr Kasznicki, Tracy Lawson
    Pages 333-353

  19. Hyperspectral Proximal Sensing for Estimating Photosynthetic Capacities at Leaf and Canopy Scales

    • Peng Fu, Christopher Montes, Katherine Meacham-Hensold
    Pages 355-372

  20. Remote Sensing Techniques: Hyperspectral Imaging and Data Analysis

    • John Stamford, Seher Bahar Aciksoz, Tracy Lawson
    Pages 373-390
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Editors and Affiliations

  • Colorado Springs, USA

    Sarah Covshoff

Bibliographic Information

  • Book Title: Photosynthesis

  • Book Subtitle: Methods and Protocols

  • Editors: Sarah Covshoff

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3790-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3789-0Published: 23 April 2024

  • Softcover ISBN: 978-1-0716-3792-0Due: 24 May 2024

  • eBook ISBN: 978-1-0716-3790-6Published: 22 April 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 471

  • Number of Illustrations: 21 b/w illustrations, 65 illustrations in colour

  • Topics: Plant Sciences

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