Abstract
Affinity purification-Mass spectroscopy (AP-MS) is a biochemical technique to identify the novel protein–protein interaction that occurs in the most relevant physiological conditions, whereas co-immunoprecipitation (Co-IP) is used to study the interaction between two known protein partners that are expressed in the native physiological conditions. Both AP-MS and Co-IP techniques are based on the ability of the interacting partners to pull-down with protein of interest. In this chapter, we have explained the AP-MS and Co-IP methods to study protein–protein interactions in the plant cells.
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Gnanasekaran, P., Pappu, H.R. (2023). Affinity Purification-Mass Spectroscopy (AP-MS) and Co-Immunoprecipitation (Co-IP) Technique to Study Protein–Protein Interactions. In: Mukhtar, S. (eds) Protein-Protein Interactions. Methods in Molecular Biology, vol 2690. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3327-4_7
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DOI: https://doi.org/10.1007/978-1-0716-3327-4_7
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3326-7
Online ISBN: 978-1-0716-3327-4
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