Abstract
Forster resonance energy transfer (FRET) is an efficient method to visualize the protein–protein interaction in living cells. This technique is based on transfer of energy between two different fluorophores that are fused to two interacting proteins. In this chapter, we described the FRET assay to visualize the protein–protein interaction in plant cells.
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References
Rios AF, Radoeva T, De Rybel B et al (2017) FRET-FLIM for visualizing and quantifying protein interactions in live plant cells. Methods Mol Biol 1497:135–146
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Gnanasekaran, P., Pappu, H.R. (2023). Forster Resonance Energy Transfer (FRET) to Visualize Protein–Protein Interactions in the Plant Cell. In: Mukhtar, S. (eds) Protein-Protein Interactions. Methods in Molecular Biology, vol 2690. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3327-4_13
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DOI: https://doi.org/10.1007/978-1-0716-3327-4_13
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3326-7
Online ISBN: 978-1-0716-3327-4
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