Abstract
In vitro cell culture models can offer high-resolution and high-throughput experimentation of cellular behaviors. However, in vitro culture approaches often fail to fully recapitulate complex cell processes involving synergistic interactions between heterogeneous neural cell populations and the surrounding neural microenvironment. Here, we describe the formation of a three-dimensional primary cortical cell culture system compatible with live confocal microscopy.
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Acknowledgments
This work was supported in part by Brown University internal research support (D.A.B.) and by the Center for Neurorestoration and Neurotechnology (N2864-C) from the United States (U.S.) Department of Veterans Affairs, Rehabilitation Research and Development Service, Providence, RI. The contents of this manuscript do not represent the views of VA or the United States Government. This work was also supported by NIA award R21AG077697 (D.A.B.) and the Brown University Carney Institute for Brain Science Zimmerman Innovation Award in Brain Science.
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Brown, S., Atherton, E., Borton, D.A. (2023). A Three-Dimensional Primary Cortical Culture System Compatible with Transgenic Disease Models, Virally Mediated Fluorescence, and Live Microscopy. In: Huang, YW.A., Pak, C. (eds) Stem Cell-Based Neural Model Systems for Brain Disorders. Methods in Molecular Biology, vol 2683. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3287-1_12
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DOI: https://doi.org/10.1007/978-1-0716-3287-1_12
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