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Cell Cultures at the Air–Liquid Interface and Their Application in Cancer Research

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Cancer Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2645))

Abstract

Air-liquid interface (ALI) cell cultures are considered a valid tool for the replacement of animals in biomedical research. By mimicking crucial features of the human in vivo epithelial barriers (e.g., lung, intestine, and skin), ALI cell cultures enable proper structural architectures and differentiated functions of normal and diseased tissue barriers. Thereby, ALI models realistically resemble tissue conditions and provide in vivo-like responses. Since their implementation, they are routinely used in several applications, from toxicity testing to cancer research, receiving an appreciable level of acceptance (in some cases a regulatory acceptance) as attractive testing alternatives to animals. In this chapter, an overview of the ALI cell cultures will be presented together with their application in cancer cell culture, highlighting the potential advantages and disadvantages of the model.

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Authors and Affiliations

  1. D3 PharmaChemistry, Nanoregulatory Group, Italian Institute of Technology, Genoa, Italy

    Luisana Di Cristo & Stefania Sabella

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  1. Luisana Di Cristo
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  2. Stefania Sabella
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Correspondence to Luisana Di Cristo .

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  1. Trinity Translational Medicine Institute, Trinity Centre for Health Sciences, Dublin, Ireland

    Dania Movia

  2. Trinity Translational Medicine Institute, Trinity Centre for Health Sciences, Dublin, Ireland

    Adriele Prina-Mello

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Di Cristo, L., Sabella, S. (2023). Cell Cultures at the Air–Liquid Interface and Their Application in Cancer Research. In: Movia, D., Prina-Mello, A. (eds) Cancer Cell Culture. Methods in Molecular Biology, vol 2645. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3056-3_2

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  • DOI: https://doi.org/10.1007/978-1-0716-3056-3_2

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