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Analysis of Plant L-Cysteine Desulfhydrase (LCD) Isozymes by Non-denaturing Polyacrylamide Gel Electrophoresis

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Plant Abiotic Stress Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2642))

Abstract

Hydrogen sulfide (H2S) is a signaling molecule that achieves different regulatory functions in animal and plant cells. The cytosolic enzyme L-cysteine desulfhydrase (LCD; EC 4.4.1.28) catalyzes the conversion of cysteine (L-Cys) to pyruvate and ammonium with the concomitant generation of H2S, this enzyme being considered one of the main sources of H2S in higher plants. Using non-denaturing polyacrylamide gel electrophoresis (PAGE) in combination with a specific assay for LCD activity, the present protocol allows identifying diverse LCD isozymes present in different organs (roots, shoots, leaves, and fruits) and plant species including pea, garlic, Arabidopsis, and pepper.

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Acknowledgments

Francisco J. Corpas and José M. Palma research is supported by a European Regional Development Fund cofinanced grant from the Spanish Ministry of Science and Innovation (PID2019-103924GB-I00) and the Plan Andaluz de Investigación, Desarrollo e Innovación (P18-FR-1359), Spain. The valuable technical help of Carmelo Ruiz-Torres is deeply appreciated. María A. Muñoz-Vargas acknowledges a Formación de Personal Investigador (FPI) predoctoral contract (PRE2020-093882) from the Spanish Ministry of Science, Innovation and Universities. Marta Rodríguez-Ruiz also acknowledges a postdoctoral contract associated to the grant P18-FR-1359. Salvador González-Gordo acknowledges an FPI contract (BES-2016-078368) from the Ministry of Economy and Competitiveness, Spain.

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Authors and Affiliations

  1. Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Stress, Development and Signaling in Plants, Estación Experimental del Zaidín, Spanish National Research Council (CSIC), Granada, Spain

    María A. Muñoz-Vargas, Marta Rodríguez-Ruiz, Salvador González-Gordo, José M. Palma & Francisco J. Corpas

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  1. María A. Muñoz-Vargas
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  2. Marta Rodríguez-Ruiz
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  3. Salvador González-Gordo
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  4. José M. Palma
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  5. Francisco J. Corpas
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Correspondence to Francisco J. Corpas .

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Editors and Affiliations

  1. UMR 6553 ECOBIO (Ecosystems-Biodiversity-Evolution), Centre National de la Recherche Scientifique (CNRS), University of Rennes, Rennes, France

    Ivan Couée

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Muñoz-Vargas, M.A., Rodríguez-Ruiz, M., González-Gordo, S., Palma, J.M., Corpas, F.J. (2023). Analysis of Plant L-Cysteine Desulfhydrase (LCD) Isozymes by Non-denaturing Polyacrylamide Gel Electrophoresis. In: Couée, I. (eds) Plant Abiotic Stress Signaling. Methods in Molecular Biology, vol 2642. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3044-0_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3044-0_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3043-3

  • Online ISBN: 978-1-0716-3044-0

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