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Modulation of superoxide dismutase (SOD) isozymes by organ development and high long-term salinity in the halophyte Cakile maritima

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Abstract

Superoxide dismutase (SOD) activity catalyzes the disproportionation of superoxide radicals into hydrogen peroxide and oxygen. This enzyme is considered to be a first line of defense for controlling the production of reactive oxygen species (ROS). In this study, the number and type of SOD isozymes were identified in the principal organs (roots, stems, leaves, flowers, and seeds) of Cakile maritima. We also analyzed the way in which the activity of these SOD isozymes is modulated during development and under high long-term salinity (400 mM NaCl) stress conditions. The data indicate that this plant contains a total of ten SOD isozymes: two Mn-SODs, one Fe-SOD, and seven CuZn-SODs, with the Fe-SOD being the most prominent isozyme in the different organs analyzed. Moreover, the modulation of SOD isozymes, particularly CuZn-SODs, was only detected during development and under severe salinity stress conditions. These data suggest that, in C. maritima, the occurrence of these CuZn-SODs in roots and leaves plays an adaptive role since this CuZn-SOD isozyme might replace the diminished Fe-SOD activity under salinity stress to overcome this adverse environmental condition.

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Acknowledgments

H. Houmani acknowledges a short-term scholarship from Tunisian government. Work in the FJ Corpas laboratory is supported by ERDF-cofinanced grant from the Ministry of Science and Innovation (BIO2012-33904) and Junta de Andalucía (group BIO192). The valuable technical help of Mr. Carmelo Ruíz-Torres is also appreciated.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080, Granada, Spain

    Hayet Houmani, Marta Rodríguez-Ruiz, José M. Palma & Francisco J. Corpas

  2. Laboratory of Extremophile Plants, Center of Biotechnology of Borj Cedria, PO Box 901, 2050, Hammam-Lif, Tunisia

    Hayet Houmani & Chedly Abdelly

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  1. Hayet Houmani
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  2. Marta Rodríguez-Ruiz
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  3. José M. Palma
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  4. Chedly Abdelly
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  5. Francisco J. Corpas
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Correspondence to Francisco J. Corpas.

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Handling Editor: Néstor Carrillo

This manuscript is dedicated to the memory of Prof. Emeritus Kozi Asada, Kyoto University (Japan) for his large contribution in the research of plant superoxide dismutase who passed away in December 15, 2013.

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Houmani, H., Rodríguez-Ruiz, M., Palma, J.M. et al. Modulation of superoxide dismutase (SOD) isozymes by organ development and high long-term salinity in the halophyte Cakile maritima . Protoplasma 253, 885–894 (2016). https://doi.org/10.1007/s00709-015-0850-1

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