Abstract
Tubulin-based polymorphism (TBP) is an intron length polymorphism (ILP) method widely applicable to any plant species and particularly suitable for a first and rapid classification of any plant genome. It is based on the selective, polymerase chain reaction (PCR)-based amplification of the two introns present at conserved positions within the coding sequences of plant β-tubulin genes. Amplification releases a simple yet distinctive genomic profile.
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Acknowledgments
The authors wish to thank the following colleagues who, during the last two decades, have worked in our lab contributing to the constant improvement of the technique: Prisca Campanoni, Mauro Bardini, Pietro Blumetti, Marcello Toschi, Andrea Altana, Claudia De Pedro, Elena Ponzoni, Anna Paola Casazza and Francesco Matromauro.
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Braglia, L., Gavazzi, F., Gianì, S., Morello, L., Breviario, D. (2023). Tubulin-Based Polymorphism (TBP) in Plant Genotyping. In: Shavrukov, Y. (eds) Plant Genotyping. Methods in Molecular Biology, vol 2638. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3024-2_28
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DOI: https://doi.org/10.1007/978-1-0716-3024-2_28
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