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Intraspecific Differentiation in White Mistletoe (Viscum album L.) Using the Analysis of Intron Length Polymorphism of β-Tubulin Genes and the SSR Analysis

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Abstract

Based on the analysis of intron I length polymorphism of the β-tubulin genes (TBP analysis), differences between two mistletoe subspecies (Viscum album ssp. austriacum (Wiesb.) and V. album ssp. album L.) have been shown. The possibility of using the Tubulin-Based Polymorphism (TBP) analysis for the sexual differentiation in mistletoe plants has also been demonstrated. We were successful in identifying particular white mistletoe genotypes using the simple sequence repeats (SSR) method. Application efficiency was comparatively assessed in both types of DNA markers, and the TBP analysis was shown to be more usable for differentiating between white mistletoe subspecies, whereas the SSR analysis is more suitable for studying genotypic variability within a particular subspecies.

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Funding

The study was supported under the approved thematics of the National Academy of Sciences of Ukraine “Population Biology and Genetics of Viscum album L. in Ukraine” (2018–2022), no. 0118U004067.

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Authors and Affiliations

  1. Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, 04123, Kyiv, Ukraine

    Yu. O. Bilonozhko, A. M. Rabokon, A. S. Postovoitova, L. O. Kalafat, S. M. Pryvalikhin, A. Ye. Demkovych, Ya. B. Blume & Ya. V. Pirko

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  1. Yu. O. Bilonozhko
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  2. A. M. Rabokon
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Correspondence to Yu. O. Bilonozhko.

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No animal or human subject participated in the experiments on which this study was based.

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Translated by N. Tarasyuk

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Bilonozhko, Y.O., Rabokon, A.M., Postovoitova, A.S. et al. Intraspecific Differentiation in White Mistletoe (Viscum album L.) Using the Analysis of Intron Length Polymorphism of β-Tubulin Genes and the SSR Analysis. Cytol. Genet. 55, 1–9 (2021). https://doi.org/10.3103/S0095452721010035

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